Koj suav li cas txwv tsis pub tawg?
Koj suav li cas txwv tsis pub tawg?

Video: Koj suav li cas txwv tsis pub tawg?

Video: Koj suav li cas txwv tsis pub tawg?
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Muab cov genome loj los ntawm cov zaus sib npaug ntawm tus lej txwv fragments ua, los yog (2.5 x 107 bp)(2.56 x 10-4 bp-1) = 6400 tawg tawg . Faib cov genome loj los ntawm tus naj npawb ntawm tawg tawg rau txiav txim qhov nruab nrab qhov loj me, lossis 2.5 x 107 bp / 6400 = 3.9 x 103 bp.]

Ntxiv rau qhov no, dab tsi txiav txim siab qhov ntev ntawm kev txwv tsis pub tawg?

Hybridization ntawm daim nyias nyias mus rau daim ntawv lo DNA soj ntsuam ces txiav txim qhov ntev ntawm tawg tawg uas yog complementary rau kev sojntsuam. A txwv tawg tawg ntev polymorphism yog hais tias tshwm sim thaum lub ntev ntawm ib qho kev kuaj pom qhov sib txawv ntawm cov tib neeg, qhia tias tsis zoo ib yam kab ke homologies.

Tsis tas li ntawd, ua li cas koj thiaj paub qhov txwv tsis pub siv enzyme? Thaum xaiv cov enzymes txwv, koj xav xaiv cov enzymes uas:

  1. Flank koj qhov ntxig, tab sis tsis txhob txiav hauv koj qhov ntxig.
  2. Nyob rau hauv qhov chaw xav tau hauv koj tus neeg tau txais plasmid (feem ntau yog nyob rau hauv Multiple Cloning Site (MCS)), tab sis tsis txhob txiav lwm qhov ntawm plasmid.

Ib yam li ntawd, tib neeg nug, dab tsi yog qhov txwv tsis pub muaj nyob hauv biology?

A txwv fragment yog DNA tawg tawg tshwm sim los ntawm kev txiav ntawm DNA strand los ntawm a txwv enzyme ( txwv endonucleases), txheej txheem hu ua txwv.

Yuav ua li cas DNA fragments tsim?

DNA muaj ob txoj hlua sib txuas ntawm nucleotides uas muab kauv ib ncig ntawm ib leeg hauv ob lub helix. Lwm yam kev txwv enzymes, xws li EcoRI, txiav los ntawm DNA strands ntawm nucleotides uas tsis raws nraim opposite ib leeg. Qhov no tsim DNA tawg nrog ib tug nucleotide strand uas overhangs thaum kawg.

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