Video: Vim li cas thiaj li txwv tsis pub txiav enzyme?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Yuav muaj ntau yam sib txawv vim li cas koj txwv enzyme tsis txiav DNA raws li kev tshuaj xyuas hauv daim video no. Kev npaj ntawm DNA yuav tsum tau cleaved yuav tsum tsis muaj cov kab mob xws li phenol, chloroform, cawv, EDTA, tshuaj ntxuav tes, lossis ntsev ntau dhau, txhua yam tuaj yeem cuam tshuam nrog txwv enzyme kev ua si.
Hauv qhov no, vim li cas kev txwv enzymes tsis txiav cov kab mob DNA?
Kab mob muaj txwv enzymes , kuj hu txwv endonucleases , qho txiav ob txoj hlua DNA ntawm cov ntsiab lus tshwj xeeb rau hauv cov khoom tawg. Txaus siab heev, txwv enzymes tsis txhob rhuav lawv tus kheej DNA . Kab mob tiv thaiv lawv tus kheej DNA los ntawm chop down txwv enzyme los ntawm methylation ntawm cov txwv qhov chaw.
Ib tug kuj yuav nug, qhov twg txwv enzymes txiav? Kev txwv Enzyme Hom Feem ntau, Hom I enzymes txiav DNA ntawm qhov chaw nyob deb ntawm qhov kev lees paub; Hom II txiav DNA nyob rau hauv lossis ze rau qhov kev lees paub; Hom III txiav DNA nyob ze ntawm kev lees paub ua ntu zus; thiab Hom IV cleave methylated DNA.
Ib yam li ntawd, nws raug nug, yuav ua li cas yog tias koj ntxiv ntau qhov txwv enzyme?
Kev zom tsis tiav yuav tshwm sim thaum ntau dhau los yog ib yam nkaus me ntsis enzyme yog siv. Cov muaj cov kab mob sib kis hauv tus DNA qauv ua tau inhibit cov enzymes , kuj ua rau digestion tsis tiav. Ib txhia txwv enzymes xav tau cofactors rau tag nrho cov dej num.
Vim li cas kuv txoj kev txwv kev zom zaub mov tsis ua haujlwm?
Tsis tiav los tsis muaj kev zom zaub mov vim kev ua haujlwm enzyme thaiv los ntawm DNA methylation. Yog tias koj lub enzyme ua haujlwm thiab digests cov tswj DNA thiab tus cov tshuaj tiv thaiv tau teeb tsa siv cov kev mob zoo, tab sis koj tseem pom teeb meem nrog kev zom zaub mov , tej zaum yog vim li cas tus enzyme yog inhibited los ntawm methylation ntawm tus DNA qauv.
Pom zoo:
Koj suav li cas txwv tsis pub tawg?
Muab cov genome loj los ntawm qhov ntau zaus sib npaug ntawm cov khoom txwv tsis pub ua, lossis (2.5 x 107 bp) (2.56 x 10-4 bp-1) = 6400 fragments. Faib cov genome loj los ntawm tus naj npawb ntawm fragments los txiav txim qhov nruab nrab fragment loj, los yog 2.5 x 107 bp / 6400 = 3.9 x 103 bp.]
Puas yog txhua qhov txwv thiab txwv tsis pub dav hlau pom hauv cov kab kos?
Txwv tsis pub Airspace yog piav qhia txog cov kab kos hauv aeronautical, suav nrog: VFR sectionals thiab IFRen-txoj kev qis qis qis. Cov ntsiab lus hais txog qhov tshwj xeeb txwv tsis pub muaj nyob rau sab ntawm cov kab ntawv sib txuas [Daim duab 3]
Yuav ua li cas kuv thiaj li txwv tsis pub kuv cov roj ntws ntsaws ntsaws?
Yuav ua li cas kho cov roj lauj kaub Leak Tshem cov roj ntsaws thiab ntws tawm tag nrho cov roj los ntawm cov roj lauj kaub rau hauv lub phiab. Muab ib tug Ntsoov Foob txwm lossis oversized ntws plug inthe ntws qhov, tshaj ib tug roj hmab O-nplhaib (gasket). Muab cov roj tshiab tso rau hauv cov roj muab tub lim hauv koj lub cav. Tshawb xyuas seb puas muaj roj xau [qhov chaw: Cortes, Sure Seal]
Kuv yuav ua li cas thiaj li txwv tsis pub kuv lub tank roj?
Yuav Ua Li Cas Txhim Kho Cov Roj Roj Tank Tso ib daim ntaub tso rau hauv pem teb kom tsis txhob muaj qhov staining ntawm qhov chaw lossis qhov chaw ua haujlwm los ntawm epoxy sealant. Muab acetone tso rau hauv lub tank thiab tig lub tank kom tshem tawm tag nrho cov kab ntawm cov roj. Muab qee cov ntsia hlau tso rau hauv lub tank thiab co nws lub zog kom tshem tawm cov kab xeb uas ua rau lub tank sab hauv
Vim li cas nws tseem ceeb los txiav cov plasmid thiab tib neeg DNA nrog tib yam kev txwv enzyme?
Cov enzymes no tseem ceeb vim tias lawv tso cai rau cov noob tshwj xeeb txiav tawm ntawm qhov chaw chromosome. Lawv kuj txiav cov kab mob plasmids. Siv tib yam kev txwv endonuclease enzyme los txiav qhib lub plasmid raws li yog siv los txiav cov noob los ntawm cov chromosome tau ua tiav cov nplaum nplaum uas tsim tawm