Vim li cas thiaj li txwv tsis pub txiav enzyme?
Vim li cas thiaj li txwv tsis pub txiav enzyme?

Video: Vim li cas thiaj li txwv tsis pub txiav enzyme?

Video: Vim li cas thiaj li txwv tsis pub txiav enzyme?
Video: Txwv tib lub xeem tsis pub sib yuav, yog vim li cas ? 2024, Kaum ib hlis
Anonim

Yuav muaj ntau yam sib txawv vim li cas koj txwv enzyme tsis txiav DNA raws li kev tshuaj xyuas hauv daim video no. Kev npaj ntawm DNA yuav tsum tau cleaved yuav tsum tsis muaj cov kab mob xws li phenol, chloroform, cawv, EDTA, tshuaj ntxuav tes, lossis ntsev ntau dhau, txhua yam tuaj yeem cuam tshuam nrog txwv enzyme kev ua si.

Hauv qhov no, vim li cas kev txwv enzymes tsis txiav cov kab mob DNA?

Kab mob muaj txwv enzymes , kuj hu txwv endonucleases , qho txiav ob txoj hlua DNA ntawm cov ntsiab lus tshwj xeeb rau hauv cov khoom tawg. Txaus siab heev, txwv enzymes tsis txhob rhuav lawv tus kheej DNA . Kab mob tiv thaiv lawv tus kheej DNA los ntawm chop down txwv enzyme los ntawm methylation ntawm cov txwv qhov chaw.

Ib tug kuj yuav nug, qhov twg txwv enzymes txiav? Kev txwv Enzyme Hom Feem ntau, Hom I enzymes txiav DNA ntawm qhov chaw nyob deb ntawm qhov kev lees paub; Hom II txiav DNA nyob rau hauv lossis ze rau qhov kev lees paub; Hom III txiav DNA nyob ze ntawm kev lees paub ua ntu zus; thiab Hom IV cleave methylated DNA.

Ib yam li ntawd, nws raug nug, yuav ua li cas yog tias koj ntxiv ntau qhov txwv enzyme?

Kev zom tsis tiav yuav tshwm sim thaum ntau dhau los yog ib yam nkaus me ntsis enzyme yog siv. Cov muaj cov kab mob sib kis hauv tus DNA qauv ua tau inhibit cov enzymes , kuj ua rau digestion tsis tiav. Ib txhia txwv enzymes xav tau cofactors rau tag nrho cov dej num.

Vim li cas kuv txoj kev txwv kev zom zaub mov tsis ua haujlwm?

Tsis tiav los tsis muaj kev zom zaub mov vim kev ua haujlwm enzyme thaiv los ntawm DNA methylation. Yog tias koj lub enzyme ua haujlwm thiab digests cov tswj DNA thiab tus cov tshuaj tiv thaiv tau teeb tsa siv cov kev mob zoo, tab sis koj tseem pom teeb meem nrog kev zom zaub mov , tej zaum yog vim li cas tus enzyme yog inhibited los ntawm methylation ntawm tus DNA qauv.

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