Hemocytometer yuav suav cov cell suav li cas?
Hemocytometer yuav suav cov cell suav li cas?

Video: Hemocytometer yuav suav cov cell suav li cas?

Video: Hemocytometer yuav suav cov cell suav li cas?
Video: Подсчет клеток с использованием видеопротокола гемоцитометра 2024, Kaum ib hlis
Anonim

Rau suav cell siv a hemocytometer ntsuas , ntxiv 15-20Μl ntawm ntawm tes suspension nruab nrab ntawm cov hemocytometer ntsuas thiab npog iav siv P-20 Pipetman. Lub hom phiaj yog kom muaj kwv yees li 100-200 hlwb / square. Suav tus tus lej ntawm hlwb nyob rau hauv tag nrho plaub lub squares faib los ntawm plaub (qhov txhais tau tias tus lej ntawm hlwb / square).

Tib neeg kuj nug tias, suav xov tooj suav li cas?

Nruab nrab siv tau cell suav per square = Tag nrho cov naj npawb siv tau hlwb nyob rau hauv 4 squares / 4. Dilution Factor = Tag Nrho Volume (Ntau ntawm cov qauv + Volume ntawm diluting kua) / Volume ntawm cov qauv. Tag nrho siv tau hlwb /Sample = Viable Cells /ml x Qhov ntim thawj ntawm cov kua los ntawm qhov ntawm tes qauv raug tshem tawm.

Tsis tas li ntawd, ua li cas koj suav cov spores siv lub hemocytometer? Cov txheej txheem suav spores siv hemocytometer:

  1. Npaj lub spore suspension.
  2. Ua tib zoo ntxuav hemocytometer thiab npog iav nrog 70% ethanol kom tsis txhob muaj kev sib kis thiab suav qhov yuam kev.
  3. Qhuav nws nrog sterilized lens ntawv.
  4. Moisten lub xub pwg nyom ntawm hemocytometer thiab txhim kho lub npog-slip siv lub siab maj mam.

Nyob rau hauv no txoj kev, koj yuav ua li cas xam cov dilution factor rau cell suav?

Rau xam tus lej ntawm hlwb koj muaj nyob rau hauv txhua tus, muab cov concentration los ntawm qhov ntim: 0.44 hlwb Luas = 13.6 mL = 6 hlwb (yog tias ua tau zoo nrog txhua tus lej tom qab). Tam sim no, rov qab mus rau diluting rau 4a: peb ntxiv 11.4mL, ua tus dilution yam : 25/11.4 = 1.84.

Tag nrho cell suav yog dab tsi?

tag nrho cell suav . tus tag nrho tus naj npawb ntawm cov nyob los yog tuag hlwb nyob rau hauv ib lub ntim los yog cheeb tsam. Rau MICROORGANISMS cov lus feem ntau yog siv rau BACTERIA, SPORES lossis YEASTS.

Pom zoo: