Video: Hemocytometer yuav suav cov cell suav li cas?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Rau suav cell siv a hemocytometer ntsuas , ntxiv 15-20Μl ntawm ntawm tes suspension nruab nrab ntawm cov hemocytometer ntsuas thiab npog iav siv P-20 Pipetman. Lub hom phiaj yog kom muaj kwv yees li 100-200 hlwb / square. Suav tus tus lej ntawm hlwb nyob rau hauv tag nrho plaub lub squares faib los ntawm plaub (qhov txhais tau tias tus lej ntawm hlwb / square).
Tib neeg kuj nug tias, suav xov tooj suav li cas?
Nruab nrab siv tau cell suav per square = Tag nrho cov naj npawb siv tau hlwb nyob rau hauv 4 squares / 4. Dilution Factor = Tag Nrho Volume (Ntau ntawm cov qauv + Volume ntawm diluting kua) / Volume ntawm cov qauv. Tag nrho siv tau hlwb /Sample = Viable Cells /ml x Qhov ntim thawj ntawm cov kua los ntawm qhov ntawm tes qauv raug tshem tawm.
Tsis tas li ntawd, ua li cas koj suav cov spores siv lub hemocytometer? Cov txheej txheem suav spores siv hemocytometer:
- Npaj lub spore suspension.
- Ua tib zoo ntxuav hemocytometer thiab npog iav nrog 70% ethanol kom tsis txhob muaj kev sib kis thiab suav qhov yuam kev.
- Qhuav nws nrog sterilized lens ntawv.
- Moisten lub xub pwg nyom ntawm hemocytometer thiab txhim kho lub npog-slip siv lub siab maj mam.
Nyob rau hauv no txoj kev, koj yuav ua li cas xam cov dilution factor rau cell suav?
Rau xam tus lej ntawm hlwb koj muaj nyob rau hauv txhua tus, muab cov concentration los ntawm qhov ntim: 0.44 hlwb Luas = 13.6 mL = 6 hlwb (yog tias ua tau zoo nrog txhua tus lej tom qab). Tam sim no, rov qab mus rau diluting rau 4a: peb ntxiv 11.4mL, ua tus dilution yam : 25/11.4 = 1.84.
Tag nrho cell suav yog dab tsi?
tag nrho cell suav . tus tag nrho tus naj npawb ntawm cov nyob los yog tuag hlwb nyob rau hauv ib lub ntim los yog cheeb tsam. Rau MICROORGANISMS cov lus feem ntau yog siv rau BACTERIA, SPORES lossis YEASTS.
Pom zoo:
Yuav ua li cas cov neeg ua haujlwm ib nrab hnub raug suav hais tias hauv kev suav nyiaj poob haujlwm?
Cov uas ua haujlwm ib ntus, ib nrab hnub, lossis puv sijhawm raug suav hais tias yog ua haujlwm, xws li cov uas ua haujlwm tsawg kawg 15 teev ntawm tsev neeg tsis them nyiaj. Txhawm rau xam cov nyiaj poob haujlwm, tus lej ntawm cov neeg poob haujlwm muab faib los ntawm cov neeg ua haujlwm, uas suav nrog txhua tus neeg ua haujlwm thiab cov neeg poob haujlwm
Cov tshuaj txav mus rau hauv thiab tawm ntawm cov cell li cas?
Cov tshuaj txav mus rau hauv thiab tawm ntawm cov hlwb los ntawm diffusion mus rau qhov concentration gradient, los ntawm ib feem permeable membrane. Kev ua tau zoo ntawm kev txav ntawm cov khoom hauv thiab tawm ntawm lub cell yog txiav txim siab los ntawm nws qhov ntim rau thaj tsam thaj tsam
Yuav ua li cas tshwm sim thaum lub concentration ntawm qabzib nyob rau hauv cov dej sab nraum lub cell yog siab tshaj qhov concentration nyob rau hauv?
Yog hais tias qhov concentration ntawm qabzib nyob rau hauv dej sab nraud ntawm lub cell yog siab tshaj qhov concentration hauv, dej yuav nyiam tawm hauv lub cell los ntawm osmosis. c. Cov piam thaj yuav zoo li nkag mus rau hauv lub cell los ntawm osmosis
Puas yog qhov kev ntsuam xyuas yuav tsum suav nrog cov txheej txheem ntsuas thiab cov txheej txheem ua haujlwm thiab cov laj thawj uas txhawb nqa Kev Tshawb Fawb Kev xav thiab kev txiav txim siab?
USPAP Standards Rule 2-2(b)(viii) xav kom tus neeg ntsuam xyuas hais hauv tsab ntawv ceeb toom txog kev soj ntsuam cov txheej txheem thiab cov txheej txheem ua haujlwm, thiab cov laj thawj uas txhawb nqa cov kev soj ntsuam, kev xav, thiab cov lus xaus; Kev cais tawm ntawm kev sib piv kev muag khoom, kev coj tus nqi lossis kev khwv nyiaj txiag yuav tsum tau piav qhia
Dab tsi yog cov cai hauv kev suav cov hlwb hauv Hemocytometer?
Kev suav cov hlwb hauv hemocytometer Thaum suav, suav tsuas yog cov hlwb ntawm cov kab ntawm ob sab ntawm lub square loj kom tsis txhob suav cov hlwb ob zaug (Daim duab 3G). Kev ncua yuav tsum tau dilute kom txaus kom cov hlwb los yog lwm yam hais tsis sib tshooj ntawm kab sib chaws, thiab yuav tsum tau faib uniformly