Hom enzyme twg cais tawm ntawm retroviruses thiab siv los tsim Cdna?
Hom enzyme twg cais tawm ntawm retroviruses thiab siv los tsim Cdna?

Video: Hom enzyme twg cais tawm ntawm retroviruses thiab siv los tsim Cdna?

Video: Hom enzyme twg cais tawm ntawm retroviruses thiab siv los tsim Cdna?
Video: Virology Lectures 2019 #9: Reverse Transcription and Integration 2024, Kaum ib hlis
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Rov qab transcriptase , kuj hu ua RNA-directed DNA polymerase , ib qho enzyme encoded los ntawm cov khoom siv caj ces ntawm retroviruses uas catalyzes transcription ntawm retrovirus RNA ( ribonucleic acid ) mus rau hauv DNA (deoxyribonucleic acid).

Hauv qhov no, dab tsi enzymes koj xav tau los ua ib lub tsev qiv ntawv cDNA?

(g) Cov enzymes hauv qab no yuav tsum tau ua kom lub tsev qiv ntawv cDNA: DNA polymerase , DNA ligase, RNA polymerase, txwv enzymes.

Tsis tas li ntawd, qhov rov qab transcriptase pom nyob qhov twg? Lawv yog pom abundantly nyob rau hauv lub genomes ntawm cov nroj tsuag thiab tsiaj. Telomerase yog lwm qhov rov qab transcriptase pom hauv ntau eukaryotes, suav nrog tib neeg, uas nqa nws tus kheej RNA template; RNA no yog siv los ua tus qauv rau DNA replication.

Tom qab ntawd, ib tug kuj nug, dab tsi yog tus qauv siv los tsim cDNA cov tsev qiv ntawv?

A cDNA Library sawv cev rau ib qho kev sau ntawm cov noob nkaus xwb uas tau nkag mus rau hauv cov protein los ntawm lub cev. Complementary DNA , los yog cDNA , yog tsim los ntawm kev thim rov qab ntawm tus tub txib RNA, thiab a tsev qiv ntawv ntawm cDNAs yog tsim los siv DNA cloning technology.

Dab tsi yog qhov thib ob enzyme siv los ua cDNA?

rov qab transcriptase

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