Cov txheej txheem:

Dab tsi yog cov cai hauv kev suav cov hlwb hauv Hemocytometer?
Dab tsi yog cov cai hauv kev suav cov hlwb hauv Hemocytometer?

Video: Dab tsi yog cov cai hauv kev suav cov hlwb hauv Hemocytometer?

Video: Dab tsi yog cov cai hauv kev suav cov hlwb hauv Hemocytometer?
Video: Kev cai hla dhau thiab kev txiav tawv yog dab tsi. 2024, Kaum ib hlis
Anonim

suav cov hlwb hauv a hemocytometer

Thaum twg suav , suav tsuas yog cov hlwb ntawm cov kab ntawm ob sab ntawm lub square loj kom zam suav cell ob zaug (Daim duab 3G). Suspensions yuav tsum tau dilute txaus kom cov hlwb los yog lwm yam hais tsis sib tshooj ntawm kab sib chaws, thiab yuav tsum tau muab faib ua tib yam.

Raws li, koj yuav suav li cas cov hlwb raug tshem tawm?

46.75 x 10,000 (104Luas = 467,500.467,500 x 5 = 2,337,500 live hlwb /ml hauv original cell ncua.

Txhawm rau xam cov naj npawb ntawm cov cell siv tau / mL:

  1. Siv qhov nruab nrab ntawm tes suav los ntawm txhua qhov ntawm 16 kaum squares.
  2. Muab 10,000 (104).
  3. Muab faib los ntawm 5 los kho rau 1: 5 dilution los ntawm Trypan Blue ntxiv.

Qhov thib ob, lub hemocytometer suav cov qe ntshav dawb li cas? A WBC suav yog ua nrog ib tug Neubauer hemocytometer . ? Siv X10 microscope magnification, suav WBC siv plaub sab loj squares nyob rau sab nrauv ntawm lub suav chav? Suav ob sab ntawm lub chamber thiab nruab nrab lub suav.

Ua li no, ua li cas koj dilute hlwb tom qab suav?

Daim ntawv qhia ib kauj ruam-ib-kauj ruam ntawm yuav ua li cas dilute hlwb

  1. Suav cov cell muaj sia. Suav tus naj npawb ntawm cov cell nyob hauv koj qhov kev npaj siv trypan xiav lossis suav cov xov tooj ntawm tes.
  2. Xam cov xov tooj uas xav tau.
  3. Ntxiv qhov suav ntim ntawm nruab nrab.
  4. Sib tov.
  5. Pipette lub cell suspension rau hauv lub phaj.

Tag nrho cell suav yog dab tsi?

tag nrho cell suav . tus tag nrho tus naj npawb ntawm cov nyob los yog tuag hlwb nyob rau hauv ib lub ntim los yog cheeb tsam. Rau MICROORGANISMS cov lus feem ntau yog siv rau BACTERIA, SPORES lossis YEASTS.

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