Yuav ua li cas yog Sanger sequencing?
Yuav ua li cas yog Sanger sequencing?

Video: Yuav ua li cas yog Sanger sequencing?

Video: Yuav ua li cas yog Sanger sequencing?
Video: Tsis Muaj Peev Xwm Yuav Ua Li Cas 2024, Kaum ib hlis
Anonim

Sanger sequencing nrog 99.99% qhov tseeb yog "tus qauv kub" rau kev tshawb fawb soj ntsuam ua ke . Txawm li cas los xij, NGS cov thev naus laus zis tshiab kuj tseem dhau los ua qhov chaw tshawb fawb tshawb fawb vim tias lawv muaj peev xwm nkag tau zoo dua thiab txo tus nqi ib tus qauv.

Kuj nug, vim li cas NGS zoo dua Sanger?

Sanger sequencing tuaj yeem ua raws li ib feem ntawm ib lub sijhawm. Vim NGS siv cov hlwb uas tuaj yeem khi ntau lab ntawm DNA, NGS tuaj yeem nyeem tag nrho cov kab ke no tib lub sijhawm. Qhov no high-throughput feature ua rau nws raug nqi heev thaum sequencing ib tug loj npaum li cas ntawm DNA.

Tsis tas li ntawd, lub ntsiab lus ntawm Sanger sequencing yog dab tsi? Sanger sequencing yog cov txheej txheem ntawm kev xaiv kev koom ua ke ntawm cov saw-txiav Dideoxynucleotides los ntawm DNA polymerase thaum lub sij hawm hauv vitro DNA replication; nws yog txoj kev siv dav tshaj plaws rau kev tshawb nrhiav SNVs.

Tom qab ntawd, ib tus tuaj yeem nug, qhov txawv ntawm Sanger sequencing thiab tiam tom ntej sequencing yog dab tsi?

tom ntej no - tiam sequencing (NGS) technologies zoo ib yam. Qhov tseem ceeb qhov sib txawv ntawm Sanger sequencing thiab NGS yog ua ke ntim. Thaum lub Sanger txoj kev nkaus xwb ua ntu zus ib qho DNA feem ntawm ib lub sij hawm, NGS yog loj heev thaum uas tig mus, ua ke tsheej lab ntawm cov tawg ib txhij ib zaug.

Yuav ua li cas chain termination sequencing ua haujlwm?

Sanger DNA ua ke tseem paub tias yog saw hlau - kev txiav txim txoj kev ntawm ua ke . ddNTPs ua rau kev txiav txim ntawm DNA strand vim ddNTPs tsis muaj 3'-OH pawg yuav tsum tau rau phosphodiester bond tsim ntawm nucleotides. Yog tsis muaj daim ntawv cog lus no, lub saw hlau ntawm nucleotides raug tsim yog xaus.

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