Video: Yuav ua li cas yog Sanger sequencing?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Sanger sequencing nrog 99.99% qhov tseeb yog "tus qauv kub" rau kev tshawb fawb soj ntsuam ua ke . Txawm li cas los xij, NGS cov thev naus laus zis tshiab kuj tseem dhau los ua qhov chaw tshawb fawb tshawb fawb vim tias lawv muaj peev xwm nkag tau zoo dua thiab txo tus nqi ib tus qauv.
Kuj nug, vim li cas NGS zoo dua Sanger?
Sanger sequencing tuaj yeem ua raws li ib feem ntawm ib lub sijhawm. Vim NGS siv cov hlwb uas tuaj yeem khi ntau lab ntawm DNA, NGS tuaj yeem nyeem tag nrho cov kab ke no tib lub sijhawm. Qhov no high-throughput feature ua rau nws raug nqi heev thaum sequencing ib tug loj npaum li cas ntawm DNA.
Tsis tas li ntawd, lub ntsiab lus ntawm Sanger sequencing yog dab tsi? Sanger sequencing yog cov txheej txheem ntawm kev xaiv kev koom ua ke ntawm cov saw-txiav Dideoxynucleotides los ntawm DNA polymerase thaum lub sij hawm hauv vitro DNA replication; nws yog txoj kev siv dav tshaj plaws rau kev tshawb nrhiav SNVs.
Tom qab ntawd, ib tus tuaj yeem nug, qhov txawv ntawm Sanger sequencing thiab tiam tom ntej sequencing yog dab tsi?
tom ntej no - tiam sequencing (NGS) technologies zoo ib yam. Qhov tseem ceeb qhov sib txawv ntawm Sanger sequencing thiab NGS yog ua ke ntim. Thaum lub Sanger txoj kev nkaus xwb ua ntu zus ib qho DNA feem ntawm ib lub sij hawm, NGS yog loj heev thaum uas tig mus, ua ke tsheej lab ntawm cov tawg ib txhij ib zaug.
Yuav ua li cas chain termination sequencing ua haujlwm?
Sanger DNA ua ke tseem paub tias yog saw hlau - kev txiav txim txoj kev ntawm ua ke . ddNTPs ua rau kev txiav txim ntawm DNA strand vim ddNTPs tsis muaj 3'-OH pawg yuav tsum tau rau phosphodiester bond tsim ntawm nucleotides. Yog tsis muaj daim ntawv cog lus no, lub saw hlau ntawm nucleotides raug tsim yog xaus.
Pom zoo:
Dab tsi yog qhov ua haujlwm ntawm DdNTP hauv DNA sequencing?
DdNTP suav nrog ddATP, ddTTP, ddCTP thiab ddGTP. DdNTP muaj txiaj ntsig zoo hauv kev tshuaj xyuas ntawm DNA cov qauv vim nws nres lub polymerization ntawm DNA strand thaum lub sij hawm DNA replication, ua qhov sib txawv ntev ntawm DNA strands replicated los ntawm ib tug template strand
PCR ua lub luag haujlwm li cas hauv Dideoxy DNA sequencing?
PCR ua lub luag haujlwm li cas hauv Dideoxy DNA sequencing? Kev siv PCR tso cai rau qib DNA synthesis los ntawm qib qis ntawm cov qauv DNA. Vim li cas kev koom ua ke ntawm dideoxynucleotide thaum lub sij hawm DNA sequencing pom tias yog ib qho kev tshwm sim 'replication-terminating'?
Vim li cas peb thiaj siv Sanger sequencing?
Sanger sequencing yog ib txoj hauv kev zoo rau kev tshawb fawb txawv txawv thaum tag nrho cov qauv qis. Rau cov kev tshawb fawb sib txawv uas tus qauv tus lej siab, amplicon sequencing nrog NGS yog qhov zoo dua thiab siv tau zoo
Dab tsi yog qhov txawv ntawm Sanger sequencing thiab tiam tom ntej sequencing?
Next-generation sequencing (NGS) technologies zoo ib yam. Qhov sib txawv tseem ceeb ntawm Sangersequencing thiab NGS yog sequencing ntim. Thaum txoj kev Sanger tsuas yog ua raws ib qho DNA ib ntus tib lub sijhawm, NGS yog qhov sib npaug loj heev, ua rau ntau lab tus kab mob ib txhij ib zaug
Vim li cas thiaj li yuav tsum tau ua kom huv PCR cov khoom ua ntej sequencing?
Kev txheeb xyuas cov khoom xav tau yog qhov tseem ceeb, uas suav nrog kev lees paub qhov tsis muaj cov khoom tsis muaj tshwj xeeb thiab primer dimers. Kev ntxuav ntawm cov tshuaj tiv thaiv sib tov kuj tseem tsim nyog los tshem tawm cov tsis muaj kev sib koom ua ke primers thiab dNTPs uas tuaj yeem cuam tshuam cov kev tshwm sim tom ntej thiab ua rau muaj qhov tsis tau nyeem