PCR ua lub luag haujlwm li cas hauv Dideoxy DNA sequencing?
PCR ua lub luag haujlwm li cas hauv Dideoxy DNA sequencing?

Video: PCR ua lub luag haujlwm li cas hauv Dideoxy DNA sequencing?

Video: PCR ua lub luag haujlwm li cas hauv Dideoxy DNA sequencing?
Video: Sanger sequencing 2024, Kaum ib hlis
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PCR ua si li cas a Lub luag haujlwm hauv Dideoxy DNA sequencing ? kev siv ntawm PCR tso cai rau detectable qib ntawm DNA synthesis los ntawm ntau qib qis ntawm template DNA . Vim li cas yog Kev koom ua ke ntawm Dideoxynucleotide thaum lub sijhawm DNA sequence txheeb xyuas qhov kev tshwm sim "replication-terminating"?

Kuj tseem paub yog, vim li cas PCR siv rau hauv cov txheej txheem DNA sequencing?

PCR sawv rau Polymerase Chain Reaction , thiab luv luv, nws luam DNA lab lub sij hawm sai heev. Nws yog siv hauv DNA sequence vim qee zaum cov DNA qauv me dhau. Qhov no tshwm sim, piv txwv li, hauv kev ua txhaum cai pov thawj, lossis hauv cov qauv qub (xws li mummies).

Ib yam li ntawd, Dideoxy sequencing yog dab tsi? DNA ua ke yog qhov kev txiav txim ntawm qhov tseeb ib ntus ntawm nucleotides hauv cov qauv ntawm DNA. Txoj kev nrov tshaj plaws rau kev ua qhov no yog hu ua ua dyoxy txoj kev los yog Sanger txoj kev (npe tom qab nws inventor, Frederick Sanger, uas tau txais 1980 Nobel nqi zog hauv chemistry [nws thib ob] rau qhov kev ua tiav).

Hais txog qhov no, lub luag haujlwm ntawm Dideoxynucleotide hauv DNA sequencing yog dab tsi?

Dideoxynucleotides yog chain-elongating inhibitors ntawm DNA polymerase, siv nyob rau hauv Sanger txoj kev rau DNA sequence . Cov Dideoxyribonucleotides tsis muaj 3 'hydroxyl pawg, yog li tsis muaj cov saw txuas ntxiv tuaj yeem tshwm sim ib zaug. dideoxynucleotide yog nyob rau ntawm txoj hlua. Qhov no tuaj yeem ua rau qhov kev txiav tawm ntawm qhov DNA sequence.

Dab tsi yog qhov ua haujlwm ntawm Ddntp hauv DNA sequencing?

a. Lawv ua raws li primers rau DNA polymerase.

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