Dab tsi yog qhov ua haujlwm ntawm DdNTP hauv DNA sequencing?
Dab tsi yog qhov ua haujlwm ntawm DdNTP hauv DNA sequencing?

Video: Dab tsi yog qhov ua haujlwm ntawm DdNTP hauv DNA sequencing?

Video: Dab tsi yog qhov ua haujlwm ntawm DdNTP hauv DNA sequencing?
Video: Протокол 5 - Подготовка к секвенированию 2024, Kaum ib hlis
Anonim

DdNTP suav nrog ddATP, ddTTP, ddCTP thiab ddGTP. DdNTP muaj txiaj ntsig hauv kev txheeb xyuas ntawm DNA cov qauv raws li nws nres lub polymerization ntawm a DNA strand thaum a DNA replication, ua qhov sib txawv ntev ntawm DNA strands replicated los ntawm ib tug template strand.

Cov lus nug tseem yog, lub luag haujlwm ntawm Dideoxynucleotide hauv DNA sequencing yog dab tsi?

Dideoxynucleotides yog chain-elongating inhibitors ntawm DNA polymerase, siv nyob rau hauv Sanger txoj kev rau DNA sequence . Cov Dideoxyribonucleotides tsis muaj 3 'hydroxyl pawg, yog li tsis muaj cov saw txuas ntxiv tuaj yeem tshwm sim ib zaug. dideoxynucleotide yog nyob rau ntawm txoj hlua. Qhov no tuaj yeem ua rau qhov kev txiav tawm ntawm qhov DNA sequence.

Tsis tas li ntawd, yuav ua li cas ddNTPs nres ib qho kev tshwm sim sequencing? Thaum muaj me me hauv ua raws li kev xav , dideoxyribonucleoside triphosphates ( ddNTPs ) terminate cov cov tshuaj tiv thaiv kab mob ntawm cov haujlwm sib txawv hauv cov DNA loj hlob. ddNTPs nres ib qho kev sib txuas lus vim lawv: ua rau DNA polymerase poob ntawm cov qauv strand. c.

Tsuas yog li ntawd, lub hom phiaj ntawm DNA sequencing yog dab tsi?

DNA sequence yog tus txheej txheem ntawm kev txiav txim siab nucleic acid ib ntus - kev txiav txim ntawm nucleotides hauv DNA . Nws suav nrog txhua txoj kev lossis thev naus laus zis uas siv los txiav txim siab txog plaub lub hauv paus: adenine, guanine, cytosine, thiab thymine.

gel electrophoresis zoo li cas hauv DNA sequencing?

Gel electrophoresis yog txheej txheem siv cais DNA fragments raws li lawv qhov loj me. DNA fragments raug nqi tsis zoo, yog li lawv txav mus rau qhov zoo electrode. Vim tag nrho DNA fragments muaj tib tus nqi ntawm tus nqi ib pawg, me me fragments txav los ntawm lub gel ceev tshaj qhov loj.

Pom zoo: