Vim li cas peb thiaj siv Sanger sequencing?
Vim li cas peb thiaj siv Sanger sequencing?

Video: Vim li cas peb thiaj siv Sanger sequencing?

Video: Vim li cas peb thiaj siv Sanger sequencing?
Video: Sanger DNA Sequencing Method 2024, Kaum ib hlis
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Sanger sequencing yog ib txoj hauv kev zoo rau cov kev tshawb fawb txawv txawv thaum tag nrho cov qauv qis. Rau cov kev tshawb fawb sib txawv uas cov qauv lej siab, amplicon ua ke nrog NGS yog qhov ua tau zoo dua thiab muaj txiaj ntsig zoo.

Ib yam li ntawd, koj tuaj yeem nug, lub hom phiaj ntawm Sanger sequencing yog dab tsi?

Sanger sequencing yog cov txheej txheem ntawm kev xaiv kev koom ua ke ntawm cov saw-txiav Dideoxynucleotides los ntawm DNA polymerase thaum lub sij hawm hauv vitro DNA replication; nws yog txoj kev siv dav tshaj plaws rau kev tshawb nrhiav SNVs.

Kuj Paub, vim li cas ddNTPs siv hauv Sanger sequencing? Dideoxynucleotides yog chain-elongating inhibitors ntawm DNA polymerase, siv hauv Sanger txoj kev rau DNA sequence . Yog li, cov molecules tsim lub hauv paus ntawm Dideoxy saw-txiav txoj kev ntawm DNA sequence , uas tau tshaj tawm los ntawm Frederick Sanger thiab nws pab neeg nyob rau hauv 1977 raws li kev txuas ntxiv ntawm kev ua haujlwm dhau los.

Kuj nug, vim li cas NGS zoo dua Sanger?

Sanger sequencing tuaj yeem ua raws li ib feem ntawm ib lub sijhawm. Vim NGS siv cov hlwb uas tuaj yeem khi ntau lab ntawm DNA, NGS tuaj yeem nyeem tag nrho cov kab ke no tib lub sijhawm. Qhov no high-throughput feature ua rau nws raug nqi heev thaum sequencing ib tug loj npaum li cas ntawm DNA.

Koj xav tau dab tsi rau Sanger sequencing?

Cov khoom xyaw rau Sanger sequencing Lawv suav nrog: A DNA polymerase enzyme. Ib tug primer, uas yog ib tug luv luv daim ntawm ib leeg-stranded DNA uas khi rau lub template DNA thiab ua raws li "pib" rau cov polymerase. Plaub DNA nucleotides (dATP, dTTP, dCTP, dGTP)

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