Dab tsi yog digestion tsis tiav?
Dab tsi yog digestion tsis tiav?

Video: Dab tsi yog digestion tsis tiav?

Video: Dab tsi yog digestion tsis tiav?
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Anonim

Ib digestive tsis tiav lub kaw lus tsuas muaj ib qho qhib. Cov khoom noj mus rau qhov qhib tib yam uas cov khib nyiab tawm. Nws yuav zoo li koj lub qhov quav yog qhov qhib tib yam li koj lub qhov ncauj! Dej nkag mus rau hauv thiab cov hlwb tshwj xeeb hu ua choanocytes lim tawm cov khoom noj.

Xav txog qhov no, vim li cas kev zom zaub mov tsis tiav tshwm sim?

Kev zom zaub mov tsis tiav yog ib qho teeb meem uas nquag ntsib thaum siv kev txwv endonucleases. Kev zom zaub mov tsis tiav muaj tshwm sim thaum siv cov enzyme ntau dhau lossis tsawg dhau. Lub xub ntiag ntawm cov kab mob hauv cov qauv DNA tuaj yeem cuam tshuam cov enzymes, kuj ua rau kev zom zaub mov tsis tiav.

Ib tug kuj tseem nug, koj tuaj yeem tso qhov kev txwv tsis pub ntev npaum li cas? *Pro-Tip* Nyob ntawm daim ntawv thov thiab tus nqi ntawm DNA nyob rau hauv cov tshuaj tiv thaiv, lub sij hawm incubation tau ntau li ntawm 45 feeb mus rau hmo ntuj. Rau kev kuaj mob zom , 1-2 hnub yog feem ntau txaus. Rau zom nrog > 1 µg ntawm DNA siv rau cloning, nws yog pom zoo tias koj digest tsawg kawg yog 4 teev.

Hauv qhov no, dab tsi yog qhov kev zom zom tag ntawm DNA?

Kev txwv Kev zom zaub mov yog cov txheej txheem ntawm kev txiav DNA molecules rau hauv me me nrog cov enzymes tshwj xeeb hu ua Restriction Endonucleases (qee zaus hu ua Restriction Enzymes lossis RE's).

Yuav ua li cas koj ua sequential digestion?

raws tu qauv rau ua ntu zus DNA Kev zom zaub mov Incubate cov tshuaj tiv thaiv ntawm kev zom zaub mov kub (feem ntau yog 37 ° C) rau 1 teev. Nres lub kev zom zaub mov los ntawm tshav kub inactivation (65 ° C rau 15 feeb) los yog ntxiv ntawm 10mM kawg concentration EDTA. Rov qab DNA siv cov khoom siv kom huv: rov muab ncua thiab dilute DNA mus rau 1 µg / µL.

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