Lub sij hawm vector hais txog dab tsi hauv genetic engineering?

2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17

Vector (molecular biology) Hauv molecular cloning, a vector yog DNA molecule siv los ua ib lub tsheb rau kev nqa txawv teb chaws caj cov ntaub ntawv mus rau lwm lub cell, qhov twg nws tau muab replicated thiab/los yog qhia (piv txwv li, plasmid, cosmid, Lambda phages). A vector muaj DNA txawv teb chaws yog hu ua recombinant DNA.

Tib neeg kuj nug, lo lus nplaum xaus hais txog dab tsi hauv gene splicing?

STICKY ENDS xa mus mus rau DNA, uas muaj Ntxiv nucleotides nyob rau hauv ib strand thiab overhangs ntxiv tshaj lwm strand. Cov nucleotides ntawm nplaum kawg yog tsis ua khub. Plasmids yog siv raws li vectors rau recombinant DNA technology. Plasmids muaj cov tshuaj tiv thaiv kab mob noob thiab ib co lwm yam kev xaiv colony nta.

Kuj Paub, dab tsi yog rau hom vectors? Rau qhov loj ntawm vectors yog:

• Plasmid. Circular extrachromosomal DNA uas autonomously replicates hauv cov kab mob cell.
• Phaj. Linear DNA molecules muab los ntawm bacteriophage lambda.
• Cosmids.
• Kab mob Artificial Chromosomes.
• Poov Artificial Chromosomes.
• Tib neeg Artificial Chromosome.

Ib yam li ntawd, qhov txawv ntawm plasmid thiab vector yog dab tsi?

Vector yog a plasmid los yog manipulated artificially tom qab ligation thiab digestion cov tshuaj tiv thaiv series, whereas a plasmid ib txwm tshwm sim hauv cov kab mob hlwb. Muaj ob peb cov duab , uas tuaj yeem siv rau hauv cov DNA recombinant, whereas tag nrho plasmids tej zaum yuav tsis siv ncaj qha rau hauv recombinant DNA technology.

Dab tsi yog qhov tseem ceeb ntawm vector?

Cov yam ntxwv tseem ceeb tshaj plaws ntawm vectors yog:

• Lub vector yuav tsum yog DNA molecule kom nws tuaj yeem cloned nrog cov noob ntawm kev txaus siab.
• Lub vector yuav tsum muaj qhov tshwj xeeb txwv qhov chaw.
• Lub vector yuav tsum muaj tus cim xaiv tau.
• Lub vector yuav tsum muaj Ori qhov chaw los ntawm qhov chaw replication tuaj yeem pib.