Video: Kev txwv enzymes siv li cas thaum ua gel electrophoresis?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
1 Teb. Txhawm rau txiav DNA, RNA, lossis plasmid ntawm txwv cov chaw (xws li EcoRI, BamHI, hindIII thiab BglII) los tsim cov caj ces me me uas tuaj yeem sib cais thiab yog li tus cwj pwm siv gel electrophoresis.
Ib yam li ib tug yuav nug, uas yog lub luag haujlwm ntawm kev txwv enzymes?
A txwv enzyme yog cov protein uas paub txog qhov tshwj xeeb, luv luv nucleotide ib ntus thiab txiav DNA tsuas yog ntawm qhov chaw tshwj xeeb, uas yog hu ua txwv qhov chaw los yog lub hom phiaj ib ntus. Hauv cov kab mob nyob, txwv enzymes ua haujlwm tiv thaiv lub cell tiv thaiv invading kab mob bacteriophages.
Tsis tas li ntawd, koj yuav ua li cas thiaj paub qhov txwv tsis pub siv enzyme? Thaum xaiv cov enzymes txwv, koj xav xaiv cov enzymes uas:
- Flank koj qhov ntxig, tab sis tsis txhob txiav hauv koj qhov ntxig.
- Nyob hauv qhov chaw xav tau hauv koj tus neeg tau txais plasmid (feem ntau yog nyob rau hauv Ntau Qhov Chaw Cloning (MCS)), tab sis tsis txhob txiav lwm qhov ntawm plasmid.
Ntxiv rau qhov no, kev txwv enzymes siv hauv kev tshawb fawb li cas?
Hauv chav kuaj, txwv enzymes (los yog txwv endonucleases ) yog siv txiav DNA rau hauv me me. Cov kev txiav yog ib txwm ua ntawm cov nucleotide ib ntus. txawv txwv enzymes lees paub thiab txiav cov DNA sib txawv.
Lub luag haujlwm ntawm gel electrophoresis yog dab tsi?
Gel electrophoresis yog cov txheej txheem siv los cais cov DNA seem raws li lawv qhov loj me. DNA cov qauv raug thauj mus rau hauv qhov dej (indentations) ntawm ib kawg ntawm a gel , thiab ib qho hluav taws xob tam sim no yog siv los rub lawv los ntawm gel . DNA tawg yog tsis zoo them, yog li lawv txav mus rau qhov zoo electrode.
Pom zoo:
Vim li cas cov kev txwv cov enzymes tseem ceeb hauv cov txheej txheem molecular?
Kev txwv cov enzymes yog cov enzymes cais los ntawm cov kab mob uas paub txog cov kab ke hauv DNA thiab tom qab ntawd txiav cov DNA los tsim cov khoom tawg, hu ua txwv tsis pub tawg. Kev txwv cov enzymes ua lub luag haujlwm tseem ceeb hauv kev tsim kho cov DNA sib txuas ua ke, raws li tau ua tiav hauv kev sim noob caj noob ces
Tus neeg siv hauv zos siv li cas thaum ya mus rau IFR txoj hauv kev?
Tus neeg siv xov tooj cua xa mus rau 'chav kawm ua ntej' thiab 'rov qab kawm' los ntawm cov kav hlau txais xov. 'Chav kawm ua ntej' yog LOC kev qhia siv los ya tus txheej txheem ILS lossis LOC txoj hauv kev. Thaum cov txheej txheem ya mus los, tus neeg nyob hauv yog nyob ntawm qhov kawg ntawm txoj kev khiav koj tab tom tsaws
Dab tsi yog qhov txwv enzymes siv rau hauv qhov xwm txheej?
Kev txwv enzyme, tseem hu ua txwv endonuclease, ib qho protein uas tsim los ntawm cov kab mob uas ua rau DNA ntawm cov chaw tshwj xeeb raws cov molecule. Nyob rau hauv cov kab mob ntawm tes, txwv enzymes cleave txawv teb chaws DNA, yog li tshem tawm cov kab mob
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Kev txwv enzymes thiab ligase siv hauv biotechnology li cas?
Kev txwv enzymes yog DNA-txiav enzymes. DNA ligase yog DNA-joining enzyme. Yog tias ob daim DNA muaj qhov sib txuam, ligase tuaj yeem txuas rau lawv los ua ib qho, tsis muaj qhov sib txawv ntawm DNA. Hauv DNA cloning, txwv enzymes thiab DNA ligase yog siv los ntxig cov noob thiab lwm yam DNA rau hauv plasmids