Vim li cas cov kev txwv cov enzymes tseem ceeb hauv cov txheej txheem molecular?
Vim li cas cov kev txwv cov enzymes tseem ceeb hauv cov txheej txheem molecular?

Video: Vim li cas cov kev txwv cov enzymes tseem ceeb hauv cov txheej txheem molecular?

Video: Vim li cas cov kev txwv cov enzymes tseem ceeb hauv cov txheej txheem molecular?
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Kev txwv cov enzymes yog cov enzymes cais tawm ntawm cov kab mob uas lees paub cov ntu tshwj xeeb hauv DNA thiab tom qab ntawd txiav cov DNA los tsim cov khoom tawg, hu ua txwv tawg. Kev txwv cov enzymes ua si heev tseem ceeb Lub luag haujlwm hauv kev tsim cov DNA recombinant molecules , raws li tau ua tiav hauv kev sim gene cloning.

Zoo ib yam tuaj yeem nug, vim li cas kev txwv cov enzymes tseem ceeb hauv kev tsim caj ces?

Kev txwv Enzymes yog cov enzymes (ib txwm pom hauv cov kab mob) uas txiav DNA ntawm cov kab DNA tshwj xeeb uas paub tias yog chaw lees paub. Txwv Enzymes yog muaj txiaj ntsig zoo hauv kev tsim noob caj noob ces raws li lawv tuaj yeem siv los txiav plasmids los tsim 'cov nplaum kawg' (qhov xaus uas raug txiav los ntawm kab zig zag, raws li qhia hauv daim duab saum toj no).

Qhov thib ob, biologists siv cov enzymes txwv rau dab tsi? Kev txwv cov enzymes yog DNA txiav cov enzymes . Txhua enzyme lees paub ib lossis ob peb lub hom phiaj ua ntu zus thiab txiav DNA ntawm lossis nyob ze cov kab ke. Hauv DNA cloning, txwv enzymes thiab DNA ligase tau siv los tso cov noob thiab lwm yam ntawm DNA rau hauv plasmids.

Ua qhov no hauv kev txiav txim siab, lub hom phiaj ntawm kev txwv enzyme yog dab tsi?

A txwv enzyme yog cov protein uas paub txog qhov tshwj xeeb, luv luv nucleotide ib ntus thiab txiav DNA tsuas yog ntawm qhov chaw tshwj xeeb, uas yog hu ua txwv qhov chaw lossis lub hom phiaj ua ntu zus. Hauv cov kab mob muaj sia, txwv kev ua haujlwm ntawm cov enzymes los tiv thaiv lub cell tiv thaiv kev kis tus kab mob bacteriophages.

Dab tsi yog hom kev txwv cov enzymes?

Kev lig kev cai, plaub hom kev txwv cov enzymes raug lees paub, raug xaiv I, II, III, thiab IV, uas txawv feem ntau hauv cov qauv, qhov chaw tawg, qhov tshwj xeeb, thiab cov cofactors.

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