Dab tsi DNA ntu ua PstI txiav?
Dab tsi DNA ntu ua PstI txiav?

Video: Dab tsi DNA ntu ua PstI txiav?

Video: Dab tsi DNA ntu ua PstI txiav?
Video: Tub ntxoov kav Shaman ntu 265 - tawm tsam kev ncaj ncees - huab tais nplog - ua rog 2024, Kaum ib hlis
Anonim

Muaj nuj nqi. PstI cov nplooj DNA ntawm kev lees paub ib ntus 5'-CTGCA / G-3' tsim cov khoom tawg nrog 3'-cohesive termini. Qhov no cleavage yields nplaum kawg 4 puag khub ntev. PstI yog catalytically active raws li ib tug dimer.

Tom qab ntawd, ib tus tuaj yeem nug, dab tsi yog qhov kev lees paub rau Psti thiab EcoRI?

EcoRI tsim 4 nucleotide nplaum xaus nrog 5' kawg overhangs ntawm AATT. Lub nucleic acid paub qhov uas cov enzyme txiav yog G/AATTC, uas muaj ib tug palindromic, complementary sequence ntawm CTTAA/G. Cov / hauv cov kab ke qhia tias cov phosphodiester daim ntawv cog lus cov enzyme yuav tawg rau hauv DNA molecule.

Ib yam li ntawd, qhov chaw txwv ntawm plasmid yog dab tsi? Qhov chaw txwv . Los ntawm Wikipedia, dawb encyclopedia. Cov chaw txwv , los yog txwv kev paub qhov chaw , yog nyob rau ntawm DNA molecule uas muaj qhov tshwj xeeb (4-8 lub hauv paus khub hauv qhov ntev) ntu ntawm nucleotides, uas tau lees paub los ntawm txwv enzymes.

Kuj paub, Psti sawv rau dab tsi?

Tus neeg mob tshwj xeeb kho kev sib hloov

BamHI nyob qhov twg?

BamHI yog hom II txwv enzyme tau los ntawm Bacillus amyloliquefaciens. Zoo li txhua hom II txwv endonucleases, nws yog ib qho dimer thiab qhov chaw lees paub yog palindromic thiab 6 lub hauv paus ntev. Nws paub txog DNA ib ntus ntawm G'GATCC thiab tawm ntawm GATC uas yog sib xws nrog ntau lwm cov enzymes.

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