Video: Dab tsi DNA ntu ua PstI txiav?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Muaj nuj nqi. PstI cov nplooj DNA ntawm kev lees paub ib ntus 5'-CTGCA / G-3' tsim cov khoom tawg nrog 3'-cohesive termini. Qhov no cleavage yields nplaum kawg 4 puag khub ntev. PstI yog catalytically active raws li ib tug dimer.
Tom qab ntawd, ib tus tuaj yeem nug, dab tsi yog qhov kev lees paub rau Psti thiab EcoRI?
EcoRI tsim 4 nucleotide nplaum xaus nrog 5' kawg overhangs ntawm AATT. Lub nucleic acid paub qhov uas cov enzyme txiav yog G/AATTC, uas muaj ib tug palindromic, complementary sequence ntawm CTTAA/G. Cov / hauv cov kab ke qhia tias cov phosphodiester daim ntawv cog lus cov enzyme yuav tawg rau hauv DNA molecule.
Ib yam li ntawd, qhov chaw txwv ntawm plasmid yog dab tsi? Qhov chaw txwv . Los ntawm Wikipedia, dawb encyclopedia. Cov chaw txwv , los yog txwv kev paub qhov chaw , yog nyob rau ntawm DNA molecule uas muaj qhov tshwj xeeb (4-8 lub hauv paus khub hauv qhov ntev) ntu ntawm nucleotides, uas tau lees paub los ntawm txwv enzymes.
Kuj paub, Psti sawv rau dab tsi?
Tus neeg mob tshwj xeeb kho kev sib hloov
BamHI nyob qhov twg?
BamHI yog hom II txwv enzyme tau los ntawm Bacillus amyloliquefaciens. Zoo li txhua hom II txwv endonucleases, nws yog ib qho dimer thiab qhov chaw lees paub yog palindromic thiab 6 lub hauv paus ntev. Nws paub txog DNA ib ntus ntawm G'GATCC thiab tawm ntawm GATC uas yog sib xws nrog ntau lwm cov enzymes.
Pom zoo:
Koj puas tuaj yeem nchuav cov pob zeb ua ntu hauv ntu?
Ncuav cov pob zeb ua ntu me me Thaum lub tsheb thauj khoom mus txog qhov kawg ntawm ntu, nthuav cov pob zeb ua kom sib npaug, thiab kov siab dua li daim ntawv, nrog rau lub laj kab tso pa/rake. Tsis txhob sau tag nrho daim foos lossis ntu ntu loj vim tias cov pob zeb ntawm cov pob zeb ntxiv koj yuav rub rov qab nrog lub rooj tsav tsheb screed yuav hnyav heev
Sanger DNA ua ntu zus ua haujlwm li cas?
Sanger sequencing tshwm sim hauv kev tsim cov khoom txuas ntxiv ntawm ntau qhov ntev txiav nrog Dideoxynucleotides ntawm 3' kawg. Tom qab ntawd cov khoom txuas ntxiv raug cais los ntawm Capillary Electrophoresis lossis CE. Cov molecules yog txhaj los ntawm ib qho hluav taws xob tam sim no rau hauv lub iav ntev capillary uas muaj gel polymer
Cov kev cai ntawm Txoj Cai Txiav Txim ntawm 1789 yog vim li cas qhov no tseem ceeb ntawm ntu 25?
Raws li Tshooj 25, Lub Tsev Hais Plaub muaj kev txiav txim rau lub xeev cov kev txiav txim siab loj tshaj plaws uas tau dhau los ntawm kev siv tau ntawm tsoomfwv txoj cai lij choj. Tshooj lus no ntawm Txoj Cai Txiav Txim Plaub Ntug ntawm 1789 tau muab lub hauv paus ntawm kev tsis sib haum xeeb thaum ntxov hauv txoj cai tswjfwm. Tom qab tsim nws txoj cai rau kev txiav txim plaub ntug hauv rooj plaub Marbury v
Vim li cas nws tseem ceeb rau kev txiav txim siab ua ntu zus ntawm cov haujlwm?
Kev Ua Si Sequencing thiab Network Diagrams. Kev ua ntu zus los tshuaj xyuas txhua yam dej num hauv WBS nrog lub hom phiaj ntawm kev txheeb xyuas kev sib raug zoo ntawm lawv thiab txheeb xyuas txhua lub sijhawm sib raug zoo ntawm cov haujlwm. Kev sib raug zoo ntawm lub sijhawm ua haujlwm yog qhov tseem ceeb vim tias lawv tswj xyuas cov haujlwm ua ntu zus thiab ua haujlwm pib thiab hnub kawg
Dab tsi yog qhov kev lees paub ua ntu zus rau PstI?
Muaj nuj nqi. PstI cleaves DNA ntawm qhov kev lees paub ib ntus 5'-CTGCA/G-3' tsim cov khoom tawg nrog 3'-cohesive termini. Qhov no cleavage yields nplaum kawg 4 puag khub ntev. PstI yog catalytically active raws li ib tug dimer