Dab tsi yog qhov kev lees paub ua ntu zus rau PstI?
Dab tsi yog qhov kev lees paub ua ntu zus rau PstI?

Video: Dab tsi yog qhov kev lees paub ua ntu zus rau PstI?

Video: Dab tsi yog qhov kev lees paub ua ntu zus rau PstI?
Video: nkauj ntseeg pua muaj leej twg paub ta yog vim dab tsi 2024, Kaum ib hlis
Anonim

Muaj nuj nqi. PstI pob DNA ntawm qhov kev lees paub ua ntu zus 5'-CTGCA/G-3' tsim cov khoom tawg nrog 3'-cohesive termini. Qhov no cleavage yields nplaum kawg 4 puag khub ntev. PstI yog catalytically active raws li ib tug dimer.

Yog li ntawd, dab tsi yog qhov kev lees paub ua ntu zus rau EcoRI?

Nws kuj yog ib feem ntawm kev txwv kev hloov kho. Hauv molecular biology nws yog siv los ua kev txwv enzyme. EcoRI tsim 4 nucleotide nplaum xaus nrog 5' kawg overhangs ntawm AATT. Cov nucleic acid kev paub ua ntu zus qhov twg cov enzyme txiav yog G/AATTC, uas muaj ib tug palindromic, complementary ib ntus of CTTAA/G.

Tsis tas li ntawd, Psti sawv rau dab tsi? Tus neeg mob tshwj xeeb kho kev sib hloov

Ntawm no, dab tsi ua rau DNA sib lawv liag ib theem kev lees paub?

A kev paub ua ntu zus yog a DNA sequence uas structural motif ntawm a DNA - kev khi domain nthuav tawm kev khi tshwj xeeb. Kev lees paub ua ntu zus yog palindromes. Txoj kev txwv endonuclease PstI lees paub, khi, thiab cleaves ib ntus 5'-CTGCAG-3'. A kev paub ua ntu zus yog txawv ntawm a qhov chaw paub.

BamHI txiav li cas?

BamHI khi ntawm qhov kev lees paub ib ntus 5'-GGATCC-3', thiab cleaves cov no ua ntu zus tsuas yog tom qab 5'-guanine ntawm txhua txoj hlua.

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