Video: Dab tsi enzymes tshem tawm ib pab pawg phosphate los ntawm lawv cov substrate?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Dephosphorylation ntiav ib hom hydrolytic enzyme, los yog hydrolase, uas cleave ester bonds. Qhov tseem ceeb hydrolase subclass siv hauv dephosphorylation yog phosphatase . Phosphatase tshem tawm phosphate pawg los ntawm hydrolysing phosphoric acid monoesters rau hauv phosphate ion thiab molecule nrog ib pab pawg dawb hydroxyl (-OH).
Tom qab ntawd, ib tus tuaj yeem nug, hom enzyme twg tshem tawm phosphate pawg los ntawm cov protein?
Phosphatase
Kuj Paub, yuav ua li cas tshwm sim thaum ATP dephosphorylated? Thaum twg ATP yog dephosphorylated , cleaving pawg phosphate tso tawm lub zog nyob rau hauv ib daim ntawv ntawm tes ua tau siv. Adenosine tsis yog tib lub hauv paus uas ua rau phosphorylation rau daim ntawv AMP, ADP, thiab ATP . Piv txwv li, guanosine kuj tuaj yeem tsim GMP, GDP, thiab GTP.
Ua raws li qhov kev pom no, dab tsi catalyzes dephosphorylation ntawm ATP rau hauv ADP?
Lub zog tso tawm los ntawm cov hluav taws xob muaj peev xwm hla lub membrane ua rau muaj enzyme, hu ua ATP synthase, ua txuas rau ADP . ATP synthase yog ib tug loj molecular complex thiab nws muaj nuj nqi yog mus catalyze qhov sib ntxiv ntawm peb pawg phosphorous los tsim ATP.
Dab tsi ntawm cov enzymes yog kinases?
Protein Kinases . Protein kinases (PTKs) yog cov enzymes uas tswj kev ua haujlwm lom neeg ntawm cov protein los ntawm phosphorylation ntawm cov amino acids tshwj xeeb nrog ATP raws li qhov chaw ntawm phosphate, yog li inducing ib tug conformational hloov los ntawm ib tug inactive mus rau ib tug active daim ntawv ntawm cov protein.
Pom zoo:
Cov neeg tshawb xyuas muaj sijhawm ntev npaum li cas tom qab hnub tshaj tawm tsab ntawv tshaj tawm los ua kom tiav cov ntaub ntawv tshawb xyuas los ntawm kev sib sau ua ke cov ntaub ntawv tshawb xyuas zaum kawg?
Cov ntaub ntawv txheeb xyuas ua tiav thiab zaum kawg yuav tsum tau muab sib dhos rau khaws cia raws li hnub tsis pub dhau 45 hnub tom qab hnub tshaj tawm daim ntawv tshaj tawm (hnub ua tiav cov ntaub ntawv)
Dab tsi yog lub npe muab rau ib feem ntawm cov enzyme uas cov substrate khi rau?
Hauv biology, thaj chaw muaj txiaj ntsig yog thaj av ntawm cov enzyme uas cov txheej txheem txheej txheej sib txuas thiab ua rau cov tshuaj tiv thaiv kab mob. Qhov chaw ua haujlwm muaj cov seem uas tsim cov ntawv cog lus ib ntus nrog cov substrate (qhov chaw khi) thiab cov khoom seem uas ua rau cov tshuaj tiv thaiv ntawm cov substrate (catalytic site)
Txoj kev twg thiaj li tshem tawm cov pa roj los ntawm cov dej pub hauv cov nroj tsuag kho dej?
Kev kho cua sov siv los cais lossis tshem tawm cov pa uas tsim nyog thiab cov impurities ntawm cov dej pub yog hu ua xyoo tom qab. Kev piav qhia: Lub deaerator yog ib qho cuab yeej siv dav siv rau kev tshem tawm cov pa oxygen thiab lwm cov pa yaj ntawm cov dej pub rau cov khoom siv hluav taws xob
Nyob rau theem twg ntawm pab tub rog tsim qauv ua pab pawg neeg pib ntseeg lawv tus kheej thiab lawv cov thawj coj?
Enrichment Stage Cov pab pawg tshiab thiab cov tswv cuab tshiab maj mam txav los ntawm kev nug txhua yam kom tso siab rau lawv tus kheej, lawv cov phooj ywg, thiab lawv cov thawj coj. Cov thawj coj kawm ntseeg los ntawm kev mloog, ua raws li lawv hnov, tsim kom muaj txoj cai meej, thiab teeb tsa cov qauv
Puas yog ib qho kev sau ntawm cov tuam txhab ywj pheej uas siv cov ntaub ntawv thev naus laus zis los tswj hwm lawv cov txiaj ntsig cov txiaj ntsig los sib sau ua khoom lag luam lossis kev pabcuam rau kev lag luam?
Lub vev xaib muaj txiaj ntsig yog ib qho kev sau ntawm cov tuam txhab ywj pheej uas siv cov ntaub ntawv thev naus laus zis los tswj hwm lawv cov txiaj ntsig kev sib koom ua ke tsim khoom lossis kev pabcuam rau kev lag luam. Ib lub tuam txhab tuaj yeem siv kev tswj hwm ntau dua ntawm nws cov neeg xa khoom los ntawm kev muaj: ntau tus neeg muag khoom