Cov txheej txheem:

Yuav ua li cas txwv enzymes manipulate DNA?
Yuav ua li cas txwv enzymes manipulate DNA?

Video: Yuav ua li cas txwv enzymes manipulate DNA?

Video: Yuav ua li cas txwv enzymes manipulate DNA?
Video: DNA Manipulative Enzyme 2024, Kaum ib hlis
Anonim

Cov kab mob siv a txwv enzyme rau tiv thaiv cov kab mob hu ua bacteriophages, lossis phages. Thaum phage kis tus kab mob, nws ntxig nws DNA mus rau hauv cov kab mob cell kom nws yuav replicated. Cov txwv enzyme tiv thaiv replication ntawm phage DNA los ntawm kev txiav nws mus rau hauv ntau daim.

Yog li ntawd, kev txwv enzymes ua dab tsi rau DNA?

Kev txwv cov enzymes . Hauv chav kuaj, txwv enzymes (los yog txwv endonucleases ) yog siv los txiav DNA mus rau hauv cov khoom me me. Cov kev txiav yog ib txwm ua ntawm cov nucleotide ib ntus. txawv txwv enzymes paub thiab txiav txawv DNA ua ntu zus.

Ib qho tseem yuav nug, vim li cas cov enzymes txwv tsis pub txiav cov kab mob DNA? Kab mob muaj txwv enzymes , kuj hu ua txwv endonucleases , uas txiav ob txoj hlua DNA ntawm cov ntsiab lus tshwj xeeb rau hauv cov khoom tawg. Txaus siab heev, txwv enzymes tsis txhob rhuav lawv tus kheej DNA . Kab mob tiv thaiv lawv tus kheej DNA los ntawm chop down txwv enzyme los ntawm methylation txwv qhov chaw.

Tsis tas li ntawd, kev txwv enzymes nrhiav thiab txiav DNA li cas?

A txwv enzyme yog a DNA - txiav enzyme uas lees paub qhov chaw tshwj xeeb hauv DNA . Ntau txwv enzymes ua staggered txiav ntawm los yog ze lawv qhov chaw paub, ua tiav nrog ib leeg-stranded overhang. Yog ob DNA molecules muaj qhov sib txuam xaus, lawv tuaj yeem koom nrog enzyme DNA ligase.

Yuav ua li cas koj xaiv txwv enzymes?

Thaum xaiv cov enzymes txwv, koj xav xaiv cov enzymes uas:

  1. Flank koj qhov ntxig, tab sis tsis txhob txiav hauv koj qhov ntxig.
  2. Nyob rau hauv qhov chaw xav tau hauv koj tus neeg tau txais plasmid (feem ntau yog nyob rau hauv Multiple Cloning Site (MCS)), tab sis tsis txhob txiav lwm qhov ntawm plasmid.

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