Cov txheej txheem:
Video: Yuav ua li cas txwv enzymes manipulate DNA?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Cov kab mob siv a txwv enzyme rau tiv thaiv cov kab mob hu ua bacteriophages, lossis phages. Thaum phage kis tus kab mob, nws ntxig nws DNA mus rau hauv cov kab mob cell kom nws yuav replicated. Cov txwv enzyme tiv thaiv replication ntawm phage DNA los ntawm kev txiav nws mus rau hauv ntau daim.
Yog li ntawd, kev txwv enzymes ua dab tsi rau DNA?
Kev txwv cov enzymes . Hauv chav kuaj, txwv enzymes (los yog txwv endonucleases ) yog siv los txiav DNA mus rau hauv cov khoom me me. Cov kev txiav yog ib txwm ua ntawm cov nucleotide ib ntus. txawv txwv enzymes paub thiab txiav txawv DNA ua ntu zus.
Ib qho tseem yuav nug, vim li cas cov enzymes txwv tsis pub txiav cov kab mob DNA? Kab mob muaj txwv enzymes , kuj hu ua txwv endonucleases , uas txiav ob txoj hlua DNA ntawm cov ntsiab lus tshwj xeeb rau hauv cov khoom tawg. Txaus siab heev, txwv enzymes tsis txhob rhuav lawv tus kheej DNA . Kab mob tiv thaiv lawv tus kheej DNA los ntawm chop down txwv enzyme los ntawm methylation txwv qhov chaw.
Tsis tas li ntawd, kev txwv enzymes nrhiav thiab txiav DNA li cas?
A txwv enzyme yog a DNA - txiav enzyme uas lees paub qhov chaw tshwj xeeb hauv DNA . Ntau txwv enzymes ua staggered txiav ntawm los yog ze lawv qhov chaw paub, ua tiav nrog ib leeg-stranded overhang. Yog ob DNA molecules muaj qhov sib txuam xaus, lawv tuaj yeem koom nrog enzyme DNA ligase.
Yuav ua li cas koj xaiv txwv enzymes?
Thaum xaiv cov enzymes txwv, koj xav xaiv cov enzymes uas:
- Flank koj qhov ntxig, tab sis tsis txhob txiav hauv koj qhov ntxig.
- Nyob rau hauv qhov chaw xav tau hauv koj tus neeg tau txais plasmid (feem ntau yog nyob rau hauv Multiple Cloning Site (MCS)), tab sis tsis txhob txiav lwm qhov ntawm plasmid.
Pom zoo:
Vim li cas cov kev txwv cov enzymes tseem ceeb hauv cov txheej txheem molecular?
Kev txwv cov enzymes yog cov enzymes cais los ntawm cov kab mob uas paub txog cov kab ke hauv DNA thiab tom qab ntawd txiav cov DNA los tsim cov khoom tawg, hu ua txwv tsis pub tawg. Kev txwv cov enzymes ua lub luag haujlwm tseem ceeb hauv kev tsim kho cov DNA sib txuas ua ke, raws li tau ua tiav hauv kev sim noob caj noob ces
Puas yog tag nrho cov kab mob muaj kev txwv enzymes?
Txwv tsis pub cov enzymes pom hauv cov kab mob (thiab lwm yam prokaryotes). Lawv lees paub thiab khi rau cov ntu tshwj xeeb ntawm DNA, hu ua chaw txwv
Dab tsi yog qhov txwv enzymes siv rau hauv qhov xwm txheej?
Kev txwv enzyme, tseem hu ua txwv endonuclease, ib qho protein uas tsim los ntawm cov kab mob uas ua rau DNA ntawm cov chaw tshwj xeeb raws cov molecule. Nyob rau hauv cov kab mob ntawm tes, txwv enzymes cleave txawv teb chaws DNA, yog li tshem tawm cov kab mob
Kev txwv enzymes thiab ligase siv hauv biotechnology li cas?
Kev txwv enzymes yog DNA-txiav enzymes. DNA ligase yog DNA-joining enzyme. Yog tias ob daim DNA muaj qhov sib txuam, ligase tuaj yeem txuas rau lawv los ua ib qho, tsis muaj qhov sib txawv ntawm DNA. Hauv DNA cloning, txwv enzymes thiab DNA ligase yog siv los ntxig cov noob thiab lwm yam DNA rau hauv plasmids
Kev txwv enzymes siv li cas thaum ua gel electrophoresis?
1 Teb. Txhawm rau txiav DNA, RNA, lossis plasmid ntawm qhov chaw txwv (xws li EcoRI, BamHI, hindIII thiab BglII) los tsim cov caj ces me me uas tuaj yeem sib cais thiab yog li tus cwj pwm siv gel electrophoresis