Video: Plasmids yog dab tsi thiab yuav siv tau li cas?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
A plasmid yog ib qho me me, extrachromosomal DNA molecule nyob rau hauv ib lub cell uas yog lub cev sib cais los ntawm chromosomal DNA thiab ua tau luam tawm ntawm nws tus kheej. Artificial plasmids yog dav siv raws li vectors hauv molecular cloning, pab rau tsav lub replication ntawm recombinant DNA sequences nyob rau hauv lub host organisms.
Ib yam li ntawd, koj tuaj yeem nug, plasmids siv rau dab tsi?
Plasmids Yog Extrachromosomal Genetic Elements Plasmids yog siv hauv genetic engineering los tsim cov DNAs recombinant thiab ua ib lub tswv yim los hloov cov noob ntawm cov kab mob. Plasmids yog "ntxiv" tus kheej-replicating genetic ntsiab pom nyob rau hauv hlwb. Lawv txawv ntawm cov chromosomes uas tsis yog qhov tseem ceeb.
Ib yam li ntawd, koj txhais li cas los ntawm plasmid? A plasmid yog ib tug me me, ncig, ob-stranded DNA molecule uas txawv ntawm ib tug cell chromosomal DNA. Plasmids ib txwm muaj nyob hauv cov kab mob hlwb, thiab lawv kuj tshwm sim hauv qee cov eukaryotes. Feem ntau, cov noob nqa hauv plasmids muab cov kab mob muaj txiaj ntsig zoo, xws li tshuaj tua kab mob.
Qhov thib ob, plasmids yog dab tsi thiab vim li cas lawv thiaj li muaj txiaj ntsig?
Cov kws tshawb fawb tuaj yeem yuam cov kab mob kom lawv. Zoo tag nrho plasmids uas yog siv los xa DNA muaj cov noob rau tshuaj tua kab mob. Thaum cov kab mob tau kho nrog a plasmid , cov kws tshawb fawb loj hlob lawv nyob rau hauv muaj cov tshuaj tua kab mob. Tsuas yog cov cell uas muaj cov plasmid yuav ciaj sia, loj hlob thiab yug me nyuam.
Yuav siv cov plasmids thiab txwv enzymes li cas?
Ob cov enzymes yog siv los tsim recombinant plasmids . Kev txwv enzymes txiav DNA ntawm qhov tshwj xeeb 4- txog 8-bp ib ntus, feem ntau tawm ntawm nws tus kheej-ua tiav ib leeg-tso tails (nplaum kawg). Cov no cov enzymes yog siv txiav ntev DNA molecules rau ntau yam txwv fragments thiab txiav a plasmid vector ntawm ib qhov chaw.
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