Cov txheej txheem:
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Rau qhov loj ntawm vectors yog:
- Plasmid. Circular extrachromosomal DNA uas autonomously replicates hauv cov kab mob cell.
- Phaj. Linear DNA molecules muab los ntawm bacteriophage lambda.
- Cosmids.
- Kab mob Artificial Chromosomes.
- Yeast Artificial Chromosomes.
- Tib neeg Artificial Chromosome.
Khaws qhov no hauv kev txiav txim siab, dab tsi yog qhov sib txawv ntawm vector?
Plaub qhov loj hom vectors yog plasmids, viral cov duab , cosmids, thiab dag chromosomes. Ntawm cov no, feem ntau siv cov duab yog plasmids. Common rau tag nrho engineered cov duab yog lub hauv paus chiv keeb ntawm replication, ib qhov chaw multicloning, thiab ib lub cim xaiv.
Tsis tas li ntawd, dab tsi yog 2 feem ntau siv vectors? Ob hom ntawm cov duab yog feem ntau siv : E. coli plasmid cov duab thiab bacteriophage λ cov duab . Plasmid cov duab replicate nrog rau lawv cov tswv cell, thaum λ cov duab replicate li lytic viruses, tua lub party cell thiab ntim cov DNA rau hauv virions (Tshooj 6).
Ntxiv mus, dab tsi yog qhov txawv ntawm prokaryotic vectors?
Cov prokaryotic vectors suav nrog plasmid muab tau cov duab , bacteriophage muab tau cov duab , pab cov duab , plam cov duab thiab fosmid cov duab . Cov no tau tham raws li hauv qab no: Plasmid Vectors : Cov no yog cov feem ntau cov duab rau cov prokaryotic cov cell.
Dab tsi yog phage vector?
Bacteriophage Lambda Vectors . Peb tau tham txog plasmids li cov duab rau cloning me me ntawm DNA. Qhov kev txwv ntawm qhov no vector yog qhov loj ntawm DNA uas tuaj yeem nkag mus rau hauv lub cell los ntawm kev hloov pauv. Qhov no nthuav tawm cov teeb meem thaum koj tab tom sim tsim lub tsev qiv ntawv genomic ntawm cov genome loj xws li nrog cov nroj tsuag.
Pom zoo:
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