Cov txheej txheem:

Yuav ua li cas koj ua ib tug dilution series?
Yuav ua li cas koj ua ib tug dilution series?
Anonim

Thawj kauj ruam hauv ua ib serial dilution yog coj ib lub ntim paub (feem ntau yog 1ml) ntawm cov Tshuag thiab muab tso rau hauv ib tug paub ntim ntawm distilled dej (feem ntau 9ml). Qhov no ua rau 10mlof dilute daws. Qhov no dilute kua muaj 1mlof extract / 10ml, ua 10-fold dilution.

Tsis tas li ntawd, koj ua li cas xam dilution?

Dilution yam muaj feem xyuam rau dilution ratios nyob rau hauv uas theDF sib npaug ntawm qhov hnyav + 1 feem

  1. Piv txwv li: Ua 300 μL ntawm 1: 250 dilution.
  2. Formula: Final Volume / Solute Volume = DF.
  3. Plug qhov tseem ceeb hauv: (300 μL) / Solute Volume = 250.
  4. Rearrange: Solute Volume = 300 μL / 250 = 1.2μL.

Ib sab saum toj, koj ua li cas 1/10 dilution? Yog li ntawd, 1:10 dilution txhais tau tias 1 feem + 9 feem ntawm dej (los yog lwm yam diluent). Piv txwv li: yog tias koj xav tau 10 mL ntawm cov 1:10 dilution Tom qab ntawd koj yuav tsum sib tov 1mL ntawm 1M NaCl nrog 9mL dej. Los yog: yog tias koj xav tau 100mL ntawm cov 1:10 kev Tom qab ntawd koj yuav tsum sib tov 10mL ntawm 1M NaCl nrog 90mL dej.

Hais txog qhov no, vim li cas serial dilution ua tiav?

A serial dilution yog ib tug series ntawm sequential dilutions siv los txo cov kab lis kev cai ntom ntom ntawm cov cell mus rau qhov siv tau ntau dua. Txhua dilution yuav txo cov concentration ntawm cov kab mob los ntawm ib tug npaum li cas.

Koj ua li cas 1/20 dilution?

Piv txwv li, a 1 : 20 dilution converts rau a 1 / 20 dilution yam. Muab qhov kawg xav tau ntim los ntawm qhov dilution qhov tseem ceeb los txiav txim qhov xav tau ntim ntawm cov khoom lag luam. Hauv peb qhov piv txwv, 30 mL x 1 ÷ 20 = 1.5 mL ntawm Tshuag tov.

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