Video: Dab tsi yog qhov chaw lees paub rau EcoRI?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Nws kuj yog ib feem ntawm kev txwv kev hloov kho. Hauv molecular biology nws yog siv los ua kev txwv enzyme. EcoRI tsim 4 nucleotide nplaum xaus nrog 5' kawg overhangs ntawm AATT. Lub nucleic kua qaub Kev lees paub ua ntu zus qhov twg cov enzyme txiav yog G / AATTC, uas muaj palindromic, ua ke ntawm CTTAA / G.
Ntxiv mus, qhov chaw lees paub rau HindIII yog dab tsi?
HindIII (hais tias "Hin D Peb") yog hom II qhov chaw - tshwj xeeb deoxyribonuclease txwv enzyme cais los ntawm Haemophilus influenzae uas cleaves DNA palindromic ib ntus AGCTTT nyob rau hauv lub xub ntiag ntawm lub cofactor Mg2+ ntawm hydrolysis.
Qhov thib ob, EcoRI thiab HindIII tau txais lawv cov npe li cas? EcoRI yog cais los ntawm E. coli hom RY13. HindIII yog tus thib peb enzyme cais los ntawm Haemophilus influenzae hom R d.
Ntxiv mus, qhov txawv ntawm EcoRI thiab HindIII yog dab tsi?
Piav koj cov lus teb. Ob qho kev txwv enzymes paub txog 6-pawg-pair ib ntus, yog li ob qho tib si yuav tsum muaj kwv yees li tib tus naj npawb ntawm qhov chaw lees paub hauv genome. Qhov loj qhov sib txawv ntawm ob yog qhov ntawd EcoRI nplooj staggered xaus, whereas SmaI nplooj blunt kawg.
Lub luag haujlwm ntawm EcoR1 yog dab tsi?
EcoR1 yog ib qho kev txwv enzyme thiab yog siv nyob rau hauv ntau yam molecular biology hom kev kawm, xws li cloning. Cov kev txwv enzymes tseem hu ua txwv endonulcease. Cov enzyme no cais tawm ntawm cov kab mob, E. coli.
Pom zoo:
Dab tsi yog qhov sib txawv ntawm ib qho chaw thiab qhov chaw ib leeg?
Nyob rau hauv kev yuav ib qho kev nrhiav tau tshwm sim thaum tsuas yog ib tus neeg muag khoom rau cov khoom yuav tsum muaj, thaum nrog ib qho kev xa khoom ib tus neeg muag khoom tshwj xeeb yog lub hom phiaj xaiv los ntawm lub koom haum yuav, txawm tias muaj lwm tus neeg xa khoom (Larson thiab Kulchitsky, 1998; Van Weele, 2010)
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