Cov kab mob hloov pauv li cas hauv chav kuaj?
Cov kab mob hloov pauv li cas hauv chav kuaj?

Video: Cov kab mob hloov pauv li cas hauv chav kuaj?

Video: Cov kab mob hloov pauv li cas hauv chav kuaj?
Video: Kub Kuaj kab mob Covid 19 puas mov rau hauv lwb rog chev 2024, Kaum ib hlis
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Kab mob tuaj yeem nqa DNA txawv teb chaws hauv cov txheej txheem hu ua kev hloov pauv . Kev hloov pauv yog ib kauj ruam tseem ceeb hauv DNA cloning. Nws tshwm sim tom qab kev txwv kev zom thiab ligation thiab hloov pauv tshiab ua plasmids rau kab mob . Tom qab kev hloov pauv , kab mob raug xaiv rau ntawm daim phiaj tshuaj tua kab mob.

Ib yam li ntawd, nws tau nug, lub hom phiaj ntawm cov kab mob hloov pauv kab mob yog dab tsi?

Cov lub hom phiaj ntawm no lab yog ua kom pom kev hloov pauv hauv cov kab mob E. coli uas tau ua hloov nrog cov noob uas sau rau cov protein fluorescent ntsuab, qhov chaw ntawm cov noob no yog bioluminescent jellyfish.

Tsis tas li ntawd, yuav ua li cas hloov cov kab mob ua rau ampicillin resistant? Cov noob tuaj yeem hloov los ntawm ib qho kab mob mus rau lwm qhov ntawm plasmid los ntawm cov txheej txheem hu ua kev hloov pauv . Hauv qhov kev sim no, plasmid nrog cov noob (DNA) rau tsis kam mus rau tshuaj tua kab mob ampicillin yuav siv los hloov cov tsis kam gene nyob rau hauv ib tug susceptible hom ntawm cov kab mob.

Tsis tas li ntawd, dab tsi yog cov kauj ruam ntawm kev hloov cov kab mob?

Ntsiab cov kauj ruam nyob rau hauv tus txheej txheem ntawm Cov kab mob hloov pauv : (1) muaj peev xwm npaj cell, (2) kev hloov pauv ntawm cell, (3) cell rov qab, thiab (4) cell plating.

Tus txheej txheem ntawm kev hloov pauv yog dab tsi?

Hauv molecular biology, kev hloov pauv yog qhov kev hloov pauv caj ces ntawm lub xov tooj ntawm tes uas tshwm sim los ntawm qhov ncaj qha uptake thiab koom ua ke ntawm exogenous genetic khoom los ntawm nws ib puag ncig los ntawm cell membrane(s).

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