Video: Cov kab mob hloov pauv li cas hauv chav kuaj?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Kab mob tuaj yeem nqa DNA txawv teb chaws hauv cov txheej txheem hu ua kev hloov pauv . Kev hloov pauv yog ib kauj ruam tseem ceeb hauv DNA cloning. Nws tshwm sim tom qab kev txwv kev zom thiab ligation thiab hloov pauv tshiab ua plasmids rau kab mob . Tom qab kev hloov pauv , kab mob raug xaiv rau ntawm daim phiaj tshuaj tua kab mob.
Ib yam li ntawd, nws tau nug, lub hom phiaj ntawm cov kab mob hloov pauv kab mob yog dab tsi?
Cov lub hom phiaj ntawm no lab yog ua kom pom kev hloov pauv hauv cov kab mob E. coli uas tau ua hloov nrog cov noob uas sau rau cov protein fluorescent ntsuab, qhov chaw ntawm cov noob no yog bioluminescent jellyfish.
Tsis tas li ntawd, yuav ua li cas hloov cov kab mob ua rau ampicillin resistant? Cov noob tuaj yeem hloov los ntawm ib qho kab mob mus rau lwm qhov ntawm plasmid los ntawm cov txheej txheem hu ua kev hloov pauv . Hauv qhov kev sim no, plasmid nrog cov noob (DNA) rau tsis kam mus rau tshuaj tua kab mob ampicillin yuav siv los hloov cov tsis kam gene nyob rau hauv ib tug susceptible hom ntawm cov kab mob.
Tsis tas li ntawd, dab tsi yog cov kauj ruam ntawm kev hloov cov kab mob?
Ntsiab cov kauj ruam nyob rau hauv tus txheej txheem ntawm Cov kab mob hloov pauv : (1) muaj peev xwm npaj cell, (2) kev hloov pauv ntawm cell, (3) cell rov qab, thiab (4) cell plating.
Tus txheej txheem ntawm kev hloov pauv yog dab tsi?
Hauv molecular biology, kev hloov pauv yog qhov kev hloov pauv caj ces ntawm lub xov tooj ntawm tes uas tshwm sim los ntawm qhov ncaj qha uptake thiab koom ua ke ntawm exogenous genetic khoom los ntawm nws ib puag ncig los ntawm cell membrane(s).
Pom zoo:
Thaum ua qhov tshuaj ntsuam xiav dawb cov kab mob hauv cov kab mob muaj?
Hloov pauv, cov kab dawb dawb tsis tuaj yeem metabolize X-Gal los tsim cov xim xiav, vim tias lawv tsis tsim cov β-galactosidase ua haujlwm tom qab noj plasmid nqa cov DNA tso thiab cuam tshuam cov lacZ α noob. Cov kab mob dawb no muaj cov kab mob recombinant thiab yuav tsum tau xaiv (Daim duab 1)
Cov kab mob chemosynthetic txawv li cas ntawm cov kab mob photosynthetic?
Cov kab mob Photosynthetic yog cov kab mob hauv cov nroj tsuag ntsuab thaum cov kab mob chemosynthetic yog saprophytes ntawm cov khoom noj uas lwj. Lub zog ntawm lub hnub ci yog siv nyob rau hauv cov kab mob photosynthetic whereas nyob rau hauv chemosynthetic kab mob lub zog yog muab los ntawm oxidation ntawm inorganic tshuaj
Txoj cai zaub mov twg tau dhau los hauv xyoo 1996 thiab hloov pauv cov tshuaj tua kab nyob rau hauv cov zaub mov tau tswj hwm li cas hauv Asmeskas?
Thaum Lub Yim Hli 1996, Thawj Tswj Hwm Clinton tau kos npe rau hauv tsab cai lij choj Food Quality Protection Act (FQPA) [16]. Txoj cai tshiab tau hloov kho Tsoom Fwv Teb Chaws Kab Kab, Fungicide, thiab Rodenticide Act (FIFRA) thiab Txoj Cai Khoom Noj, Tshuaj, thiab Tshuaj pleev ib ce (FDCA), hloov pauv txoj hauv kev EPA tswj cov tshuaj tua kab
Dab tsi yog tus neeg sawv cev ntawm kev hloov pauv hauv cov kab mob?
Hauv cov kab mob, kev hloov pauv yog ua los ntawm kev sib xyaw transgenic DNA nrog cov kab mob hlwb kho kom lawv muaj peev xwm coj tau DNA. Ntau yam ntawm lwm txoj kev tau siv los qhia DNA rau hauv cov nroj tsuag thiab tsiaj txhu xws li DNA txhaj, electroporation, thiab microparticle bombardment
Yuav ua li cas tom qab cov kab mob hloov pauv?
Cov kab mob tuaj yeem nqa cov DNA txawv teb chaws hauv cov txheej txheem hu ua kev hloov pauv. Kev hloov pauv yog ib kauj ruam tseem ceeb hauv DNA cloning. Nws tshwm sim tom qab kev txwv kev zom thiab ligation thiab hloov cov plasmids tshiab rau cov kab mob. Tom qab kev hloov pauv, cov kab mob raug xaiv rau ntawm daim phiaj tshuaj tua kab mob