Thaum ua qhov tshuaj ntsuam xiav dawb cov kab mob hauv cov kab mob muaj?
Thaum ua qhov tshuaj ntsuam xiav dawb cov kab mob hauv cov kab mob muaj?

Video: Thaum ua qhov tshuaj ntsuam xiav dawb cov kab mob hauv cov kab mob muaj?

Video: Thaum ua qhov tshuaj ntsuam xiav dawb cov kab mob hauv cov kab mob muaj?
Video: tshuaj pujkaum kho pojniam lub qhov nqaij tom qab yug menyuam tag 2024, Kaum ib hlis
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Conversely, cov dawb colonies tsis tuaj yeem hloov pauv X-Gal los tsim cov tshuaj xiav xim, vim tias lawv tsis tsim cov kev ua haujlwm β-galactosidase tom qab noj plasmid nqa cov DNA tso thiab cuam tshuam rau lacZ α noob. Cov no dawb colonies muaj tus recombinant kab mob thiab yuav tsum tau xaiv (Daim duab 1).

Ua li no, puas yog txhua pawg neeg dawb thiab xiav muaj plasmid?

Cov xiav colonies muaj "self" religated plasmids tias ua tsis muaj DNA nkag mus cuam tshuam rau lac Z gene. Dawb colonies muaj cov kab mob uas nqa plasmids uas muaj DNA ntxig cov khoom uas cuam tshuam cov lac Z gene. Kev xaiv yuav ua tiav ntawm ampicillin muaj nruab nrab

Ib yam li ntawd, lub hom phiaj ntawm kev tshuaj ntsuam xiav dawb yog dab tsi? Cov xiav – dawb screen yog a tshuaj ntsuam cov txheej txheem uas tso cai rau kev txheeb xyuas sai thiab yooj yim ntawm cov kab mob recombinant hauv vector-based molecular cloning thwmsim. DNA ntawm kev txaus siab yog ligated rau hauv vector.

Xav txog qhov no, qhov kev tshuaj ntsuam xiav dawb pawg neeg dawb yog dab tsi?

Cov xiav - dawb screen yog a kev tshuaj ntsuam cov txheej txheem uas tso cai rau kev tshawb pom ntawm kev vam meej ligations hauv vector-based gene cloning. DNA ntawm kev txaus siab tau txuas rau hauv vector. Yog tias ligation tau ua tiav, cov kab mob pawg yuav yog dawb ; yog tsis yog, cov pawg yuav yog xiav.

Vim li cas qee lub nroog xiav thiab lwm tus yog dawb?

Ib qho pawg muaj plasmid (thiab yog li ntawd ua haujlwm gene-galactosidase gene) yuav tig mus xiav , los ntawm kev ua haujlwm β-galactosidase. Qhov no hu ua α-complementation. Qhov ntxig cuam tshuam rau gene-galactosidase noob, thiab yog li ntawd cov no cov cheeb tsam nyob twj ywm dawb.

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