Yuav ua li cas yog tias koj ntxiv ntau yam kev txwv enzyme?
Yuav ua li cas yog tias koj ntxiv ntau yam kev txwv enzyme?

Video: Yuav ua li cas yog tias koj ntxiv ntau yam kev txwv enzyme?

Video: Yuav ua li cas yog tias koj ntxiv ntau yam kev txwv enzyme?
Video: Yuav ua cas yog tswv Yexus los tos cov ntseeg mus lawm tas tshuav koj nyob 2024, Kaum ib hlis
Anonim

Kev zom tsis tiav yuav tshwm sim thaum ntau dhau los yog ib yam nkaus me ntsis enzyme yog siv. Cov muaj cov kab mob sib kis hauv tus DNA qauv ua tau inhibit cov enzymes , kuj ua rau digestion tsis tiav. Ib txhia txwv enzymes xav tau cofactors rau tag nrho cov dej num.

Tsis tas li ntawd, vim li cas thiaj li txwv tsis pub enzymes ntxiv zaum kawg?

Cov txwv enzyme feem ntau yog cov kawg tiv thaiv ntxiv mus rau ib qho tshuaj tiv thaiv kom ntseeg tau tias nws tsis raug rau cov xwm txheej hnyav. Cov tshuaj tiv thaiv sib sau ua ke yuav tsum sib xyaw tom qab enzyme ntxiv. Sib tov tag nrho cov tshuaj uas muaj txwv enzymes maj mam zam enzyme kev tsis ua haujlwm.

Tsis tas li ntawd, yuav ua li cas txwv kev zom zaub mov kom zoo dua? Tsis txhob siv ntau tshaj qhov pom zoo enzyme tus nqi (piv txwv li, 10 units ntawm enzyme ib microgram ntawm DNA). Txo tus nqi ntawm enzyme hauv cov tshuaj tiv thaiv, yog tias tsim nyog. Tsis txhob ntev incubation ntawm lub kev zom zaub mov tshuaj tiv thaiv. Siv cov tshuaj tiv thaiv tsis zoo.

Kuj paub, vim li cas thiaj yuav txwv tsis pub txiav enzyme?

Kev npaj ntawm DNA yuav tsum tau cleaved yuav tsum tsis muaj cov kab mob xws li phenol, chloroform, cawv, EDTA, tshuaj ntxuav tes, lossis ntsev ntau dhau, txhua yam tuaj yeem cuam tshuam nrog txwv enzyme kev ua si. Yog tias muaj cov tshuaj inhibitor (feem ntau ntsev, EDTA lossis phenol) tam sim no, tus tswj DNA yuav tsis txiav tom qab sib tov.

Vim li cas cov enzyme tuaj yeem ua rau lub hnub qub ua haujlwm?

Lub hnub qub kev ua si yog kev so lossis kev hloov pauv ntawm qhov tshwj xeeb ntawm kev txwv enzyme mediated cleavage ntawm DNA uas ua tau tshwm sim nyob rau hauv cov tshuaj tiv thaiv tej yam kev mob uas txawv heev ntawm cov zoo rau cov enzyme . Lub hnub qub kev ua si tuaj yeem tshwm sim vim muaj Mg2+, as yog pom hauv HindIII, piv txwv li.

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