Video: Koj puas tuaj yeem ua raws li PCR khoom?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Ncaj qha Sequencing ntawm PCR cov khoom . Nws yog qhov ua tau ncaj qha ib ntus ib PCR khoom yam tsis muaj thawj cloning lub fragment. Ncaj qha PCR sequencing tsis tshua muaj kev vam meej tshwj tsis yog koj siv qee lub sijhawm kom ntseeg tau tias koj tsis poob rau hauv ib ntawm ntau cov ntxiab.
Hauv qhov no, PCR sequencing yog dab tsi?
Ncaj qha Sequencing ntawm PCR Cov khoom. Koj yuav tsum tshem tawm tag nrho PCR primers thiab unincorporated nucleotides ua ntej cov khoom yog ua ke . Sequencing siv tsuas yog ib qho primer es tsis txhob siv ob qho tib si hauv PCR . Yog tias koj tsis tshem ob lub primers, koj yuav tau txais ob ua ntu zus superimposed rau ib leeg uas nyeem tsis tau.
Ib yam li ntawd, kuv puas tuaj yeem siv PCR primers rau sequencing? PCR primers yog npaj rau PCR amplification kom tau ib qho amplicon. Sequencing primers yog siv rau ib ntus DNA tawg thiab nthuav tawm nws cov DNA ib ntus txheeb xyuas. 4. Ob PCR primers xav tau nyob rau hauv ib PCR tshuaj tiv thaiv (feem ntau); ib leeg xwb sequencing primer yog ntxiv rau a ua ke tshuaj tiv thaiv.
Ua raws li qhov no, koj yuav ua li cas kuaj cov khoom PCR?
Nrhiav thiab tsom xam ntawm PCR cov khoom . Cov khoom ntawm a PCR yuav tsum yog ib feem lossis tawg ntawm DNA ntawm qhov ntev. Ntau cov tswv yim tuaj yeem siv los txheeb xyuas cov kab ke sib txuas (saib Table). Cov txheej txheem yooj yim tshaj plaws thiab feem ntau siv yog electrophoresis ntawm cov PCR khoom ntawm agarose gel nrog EtBr (ethidium bromide).
Vim li cas thiaj li yuav tsum tau ua kom huv PCR cov khoom ua ntej sequencing?
Kev huv huv DNA los ntawm a PCR cov tshuaj tiv thaiv yog feem ntau tsim nyog rau siv downstream, thiab pab kom tshem tawm cov enzymes, nucleotides, primers thiab tsis khoom.
Pom zoo:
Puas yog cov txheej txheem uas hloov cov tswv yim rau hauv cov txiaj ntsig uas tuaj yeem raug muag raws li cov khoom lag luam thiab cov kev pabcuam?
Kev tswj hwm kev ua haujlwm hloov pauv cov tswv yim (kev ua haujlwm, peev txheej, khoom siv, av, vaj tse, khoom siv, thiab cov ntaub ntawv) rau hauv cov khoom (khoom thiab kev pabcuam) uas muab tus nqi ntxiv rau cov neeg siv khoom. Txhua lub koom haum yuav tsum siv zog ua kom zoo tshaj plaws ntawm lawv cov txheej txheem hloov pauv kom tau raws li cov neeg siv khoom xav tau
Tus neeg yuav khoom tuaj yeem ua dab tsi raws li UCC yog tias nws xa khoom tsis raug?
Raws li Txoj Cai Kev Lag Luam Uniform (UCC), yog tias tus neeg muag khoom xa cov khoom tsis haum, tus neeg yuav khoom tuaj yeem tsis lees paub tag nrho cov khoom, lees txais tag nrho cov khoom, lossis lees txais qee yam thiab tsis lees txais cov khoom seem. Kev tsis lees paub cov khoom tsis haum yuav tsum tau ua los ntawm tus neeg yuav khoom hauv lub sijhawm tsim nyog tom qab cov khoom xa tuaj
Cov khoom muag puas tuaj yeem muag raws li kev muag khoom?
Txoj kev muag khoom tsis tuaj yeem siv rau hauv cov xwm txheej hauv qab no: Kev muag khoom ntawm cov khoom muag hauv kev lag luam ib txwm muaj, txawm tias tus neeg siv khoom them rau cov khoom muag hauv ib xyoos tom qab. Kev muag khoom ntawm tus kheej lossis cov khoom ntiag tug los ntawm cov tswv lag luam, txawm tias cov cuab yeej raug muag ntawm qhov kev npaj them nqi
Koj puas tuaj yeem muab cov khoom sib xyaw ua ke nrog cov khoom siv tag nrho?
Yog li, yog, koj tuaj yeem sib xyaw cov roj hluavtaws thiab cov pa roj kom zoo. Nyob rau hauv qhov tseeb, hluavtaws-blend lub cev muaj zog roj tsuas yog cov pa thiab hluavtaws roj twb tov rau koj. Tab sis, txwv tsis pub muaj xwm txheej ceev, nws tsis yog lub tswv yim zoo
Koj puas tuaj yeem muag koj daim pib dav hlau uas tsis tuaj yeem thim rov qab?
Hom daim pib Koj tseem tuaj yeem muag daim npav mus ncig, uas yog cov qhab nia uas tau muab los ntawm dav hlau yog tias koj tau raug tsoo los ntawm lub davhlau (txawm tias lub dav hlau tsis yog lub suab uas tso cai hloov lub npe ntawm daim pib). Nws tso cai rau koj muag ib feem ntawm cov nyiaj rov qab cov tsev so tshwj xeeb, tsuav yog ib feem ntawm nws tsis tuaj yeem thim rov qab