Koj puas tuaj yeem ua raws li PCR khoom?

2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17

Ncaj qha Sequencing ntawm PCR cov khoom . Nws yog qhov ua tau ncaj qha ib ntus ib PCR khoom yam tsis muaj thawj cloning lub fragment. Ncaj qha PCR sequencing tsis tshua muaj kev vam meej tshwj tsis yog koj siv qee lub sijhawm kom ntseeg tau tias koj tsis poob rau hauv ib ntawm ntau cov ntxiab.

Hauv qhov no, PCR sequencing yog dab tsi?

Ncaj qha Sequencing ntawm PCR Cov khoom. Koj yuav tsum tshem tawm tag nrho PCR primers thiab unincorporated nucleotides ua ntej cov khoom yog ua ke . Sequencing siv tsuas yog ib qho primer es tsis txhob siv ob qho tib si hauv PCR . Yog tias koj tsis tshem ob lub primers, koj yuav tau txais ob ua ntu zus superimposed rau ib leeg uas nyeem tsis tau.

Ib yam li ntawd, kuv puas tuaj yeem siv PCR primers rau sequencing? PCR primers yog npaj rau PCR amplification kom tau ib qho amplicon. Sequencing primers yog siv rau ib ntus DNA tawg thiab nthuav tawm nws cov DNA ib ntus txheeb xyuas. 4. Ob PCR primers xav tau nyob rau hauv ib PCR tshuaj tiv thaiv (feem ntau); ib leeg xwb sequencing primer yog ntxiv rau a ua ke tshuaj tiv thaiv.

Ua raws li qhov no, koj yuav ua li cas kuaj cov khoom PCR?

Nrhiav thiab tsom xam ntawm PCR cov khoom . Cov khoom ntawm a PCR yuav tsum yog ib feem lossis tawg ntawm DNA ntawm qhov ntev. Ntau cov tswv yim tuaj yeem siv los txheeb xyuas cov kab ke sib txuas (saib Table). Cov txheej txheem yooj yim tshaj plaws thiab feem ntau siv yog electrophoresis ntawm cov PCR khoom ntawm agarose gel nrog EtBr (ethidium bromide).

Vim li cas thiaj li yuav tsum tau ua kom huv PCR cov khoom ua ntej sequencing?

Kev huv huv DNA los ntawm a PCR cov tshuaj tiv thaiv yog feem ntau tsim nyog rau siv downstream, thiab pab kom tshem tawm cov enzymes, nucleotides, primers thiab tsis khoom.