Dab tsi yog txhais los ntawm enzyme immobilization?
Dab tsi yog txhais los ntawm enzyme immobilization?

Video: Dab tsi yog txhais los ntawm enzyme immobilization?

Video: Dab tsi yog txhais los ntawm enzyme immobilization?
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Ib immobilized enzyme yog ib enzyme txuas mus rau ib qho inert, insoluble khoom-xws li calcium alginate (tsim los ntawm reacting ib tug sib tov ntawm sodium alginate tov thiab enzyme daws nrog calcium chloride). Qhov no tuaj yeem ua rau muaj zog tiv thaiv kev hloov pauv hauv cov xwm txheej xws li pH lossis kub.

Yog li ntawd, dab tsi yog txhais los ntawm immobilization?

: kev act immobilizing los yog lub xeev ntawm kev ua immobilized :as. a: nyob ntsiag to so hauv txaj rau lub sij hawm ntev siv los kho cov kab mob (raws li tuberculosis) b: fixation (raws li los ntawm plaster cam khwb cia) ntawm ib feem ntawm lub cev feem ntau los txhawb kev kho nyob rau hauv ib txwm muaj kev sib raug zoo.

Ib yam li ntawd, enzyme immobilization cuam tshuam li cas rau enzyme kinetics? Immobilisation ua tau zoo heev nyhuv stability ntawm ib enzyme . Yog tias immobilisation txheej txheem qhia txhua yam strain rau hauv enzyme , qhov no yuav txhawb kom inactivation ntawm lub cov enzymes nyob rau hauv denaturing tej yam kev mob (piv txwv li, siab dua kub los yog heev ntawm pH).

Raws li txoj cai, cov txheej txheem ntawm enzyme immobilization yog dab tsi?

Kev lig kev cai, plaub txoj kev yog siv rau enzyme immobilization , uas yog (1) non-covalent adsorption thiab deposition, (2) lub cev entrapment, (3) covalent attachment, thiab (4) bio-conjugation (Fig. 2). Kev txhawb nqa kev khi tuaj yeem yog lub cev lossis tshuaj lom neeg, suav nrog cov nyiaj tsis muaj zog lossis covalent bonds.

Vim li cas immobilized enzymes ruaj khov dua?

Qhov nce ruaj khov tej zaum yuav tshwm sim los ntawm qhov tseeb tias immobilization txwv lub thermal txav ntawm lub enzyme ntawm qhov kub siab dua. Yog li ntawd, thermal denaturation yuav tsis tshwm sim ntawm qhov kub siab dua nrog ib qho immobilized enzyme.

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