Vim li cas DNA microarray yog ib qho cuab yeej tseem ceeb?
Vim li cas DNA microarray yog ib qho cuab yeej tseem ceeb?

Video: Vim li cas DNA microarray yog ib qho cuab yeej tseem ceeb?

Video: Vim li cas DNA microarray yog ib qho cuab yeej tseem ceeb?
Video: DNA Microarray (DNA chip) technique 2024, Kaum ib hlis
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DNA microarray yog qhov ua tau zoo cuab yeej hauv transcriptomics uas pab peb hauv kev kawm thiab tshuaj xyuas mRNA qhia ntawm yuav luag txhua cov noob tam sim no hauv lub cev. Nrog rau qhov muaj tag nrho-genome sequencing ntawm microorganisms, tam sim no tau dhau los ua kom paub cov noob uas muaj peev xwm rau bioremediation.

Tom qab ntawd, ib tus kuj yuav nug, vim li cas DNA microarray siv?

A DNA microarray (tseem hu ua DNA chip los yog biochip) yog ib phau ntawm microscopic DNA cov chaw txuas mus rau qhov chaw ruaj khov. Cov kws tshawb fawb siv DNA microarrays ntsuas cov theem qhia ntawm ntau cov noob ib txhij los yog mus rau genotype ntau thaj tsam ntawm ib lub genome.

Kuj Paub, vim li cas microarray yuav tsum tau ntxuav? DNA microarrays tuaj yeem ua tau siv los txiav txim qhov kev qhia ntawm ntau cov noob hauv ib qho tshuaj tiv thaiv. Tom qab hybridization, lub microarray yog ntxuav txhawm rau tshem tawm cov kev sib khi tsis tshwj xeeb thiab tshuaj ntsuam xyuas kom ntsuas cov fluorescence ntawm txhua qhov chaw.

Kuj tseem paub yog, DNA microarray ua haujlwm li cas?

Lub hauv paus ntsiab lus qab microarrays yog hais tias complementary sequences yuav khi rau ib leeg. Qhov tsis paub DNA molecules raug txiav mus rau hauv cov khoom tawg los ntawm kev txwv endonucleases; Cov cim fluorescent txuas nrog rau cov no DNA tawg. Cov no tau tso cai rau hnov mob nrog kev soj ntsuam ntawm cov DNA nti.

Dab tsi yog qhov txwv ntawm DNA microarray technology?

qib siab yav dhau los vim yog hla kev sib xyaw; txwv tsis pub muaj ntau yam kev tshawb nrhiav vim yog ob qho tib si keeb kwm yav dhau thiab cov cim saturation; Kev sib piv cov theem qhia thoob plaws cov kev sim sib txawv feem ntau nyuaj thiab tuaj yeem xav tau cov txheej txheem normalization nyuaj.

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