Video: Dab tsi yog plasmid insert?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
A plasmid yog ib tug me me, ncig, ob-stranded DNA molecule uas txawv ntawm ib tug cell chromosomal DNA. Cov kws tshawb fawb tuaj yeem ntxig DNA fragments los yog noob rau hauv a plasmid vector, tsim ib tug thiaj li hu ua recombinant plasmid . Qhov no plasmid tuaj yeem nkag mus rau hauv cov kab mob los ntawm txoj kev hu ua transformation.
Yog li ntawd, plasmid yog dab tsi thiab nws txoj haujlwm yog dab tsi?
Kev ua haujlwm ntawm Plasmid Plasmids muaj ntau yam sib txawv muaj nuj nqi . Lawv tuaj yeem muaj cov noob uas txhim kho txoj sia nyob ntawm lub cev, los ntawm kev tua lwm yam kabmob lossis los ntawm kev tiv thaiv tus tswv ntawm tes los ntawm kev tsim cov co toxins. Ib txhia plasmids pab txhawb cov txheej txheem ntawm replication nyob rau hauv cov kab mob.
Ib sab saum toj no, dab tsi yog ntxig DNA? Hauv Molecular biology, ib ntxig yog ib feem ntawm DNA yog ntxig mus rau hauv ib qho loj DNA vector los ntawm recombinant DNA txheej txheem, xws li ligation los yog recombination. Qhov no tso cai rau nws yuav muab ntau ntxiv, xaiv, ntxiv manipulated los yog qhia nyob rau hauv ib tug tswv tsev kab mob.
Kuj tseem paub yog, cov noob tau muab tso rau hauv plasmid li cas?
Hauv kev sim cloning ib txwm, lub hom phiaj noob yog muab tso rau hauv ib ncig ntawm DNA hu ua a plasmid . Cov plasmid tau qhia rau hauv cov kab mob ntawm cov txheej txheem hu ua transformation, thiab cov kab mob nqa cov plasmid raug xaiv siv tshuaj tua kab mob.
Lub hom phiaj ntawm ligation yog dab tsi?
Hauv molecular biology, ligation hais txog kev koom ua ke ntawm ob qho DNA tawg los ntawm kev tsim cov phosphodiester daim ntawv cog lus. Hauv chav kuaj, DNA ligase yog siv thaum lub sij hawm molecular cloning los koom DNA fragments ntawm inserts nrog vectors - carrier DNA molecules uas yuav replicate lub hom phiaj fragments nyob rau hauv cov kab mob.
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