Video: Vim li cas NGS zoo dua Sanger?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Qauv loj
NGS yog pheej yig dua, nrawm dua, xav tau DNA tsawg dua thiab yog ntau raug thiab txhim khu kev qha tshaj Sanger ua ke. Cia peb saib qhov no ntau nyob ze. Rau Sanger sequencing, ib tug loj npaum li cas ntawm templateDNA yog xav tau rau txhua tus nyeem
Cov neeg kuj nug, dab tsi yog qhov zoo ntawm cov tiam tom ntej sequencing?
Txhua tus muaj qhov tshwj xeeb qhov zoo rau cov txheej txheem: nyeem ntawv, qhov tseeb, lub sijhawm ua haujlwm, thiab dhau mus. Ntxiv nrog rau kev txheeb xyuas DNA ntu, kev nce qib ntawm ua ke Cov thev naus laus zis tau ua rau kev soj ntsuam ntawm lwm yam khoom siv lom neeg xws li RNA thiab cov protein, nrog rau lawv cuam tshuam li cas hauv cov cellular networks nyuaj.
Ib tug kuj yuav nug, vim li cas Sanger sequencing tseem siv? Sanger sequencing txhim kho ntau xyoo dhau los, feem ntau vim yog automation, thiab yog lub hauv paus rau ua ke thawj tib neeg genome hauv 2000. Hauv tsawg dua ob xyoos, txawm li cas los xij, NGS tau dhau los Sanger sequencing vim nws high throughput, parallel ua haujlwm, thiab ntau tus nqi qis perbase.
Raws li, qhov sib txawv ntawm Sanger sequencing thiab tiam tom ntej sequencing yog dab tsi?
Txhua qhov sib xyaw nucleotide raug txheeb xyuas los ntawm nws cov fluorescent tag. Qhov tseem ceeb qhov sib txawv ntawm Sangersequencing thiab NGS yog ua ke ntim. Thaum lub Sanger txoj kev nkaus xwb ua ntu zus Ib qho DNA ib feem ntawm lub sijhawm, NGS yog qhov sib npaug loj heev, ua ke lab offragments ib txhij ib zaug.
Yuav ua li cas yog Sanger sequencing?
Sanger sequencing nrog 99.99% qhov tseeb yog "tus qauv kub" rau kev tshawb fawb soj ntsuam ua ke . Txawm li cas los xij, cov thev naus laus zis tshiab NGS kuj tseem muaj nyob hauv cov chaw tshawb fawb soj ntsuam vim lawv muaj peev xwm ua tau ntau dua thiab cov nqi qis dua rau ib tus qauv.
Pom zoo:
Vim li cas kev txhawb siab sab hauv zoo dua?
Kev txhawb siab sab hauv txhawb kom muaj kev sib koom ua ke thiab qib siab dua ntawm kev rau siab thiab ua haujlwm ntev (Pinder 2011). Qhov tseeb, kev tshawb fawb tsis ntev los no tau pom tias kev mob siab rau hauv lub cev tuaj yeem ua haujlwm tau zoo hauv kev ua haujlwm zoo li cov khoom plig extrinsic hauv kev kawm thiab chaw ua haujlwm (Cerasoli li al
Dab tsi yog qhov sib txawv ntawm ib qho kev xa khoom thiab ntau qhov kev xa khoom uas zoo dua yog vim li cas?
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Vim li cas cov chiv hluavtaws zoo dua li cov chiv chiv?
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Vim li cas qhov khoom plig zoo tshaj plaws yog qhov khoom plig zoo dua li extrinsic?
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Vim li cas margin zoo dua li markup?
Qhov sib txawv ntawm margin thiab markup yog tias margin yog muag rho tawm tus nqi ntawm cov khoom muag, thaum markup yog tus nqi uas tus nqi ntawm cov khoom yog nce nyob rau hauv thiaj li yuav muab tus nqi muag. Los yog, teev raws li qhov feem pua, qhov feem pua ntawm cov paj yog 30% (xws li cov paj tau faib los ntawm kev muag khoom)