Video: BamHI puas tuaj yeem ua kom tsis muaj cua sov?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Txawm tias NdeI tau ua kub inactivated , BamHI -HF tsis tuaj yeem.
Ua li no, ua li cas koj inactivate BamHI?
Kub kev tsis ua haujlwm tau ua raws li hauv qab no kom kwv yees ib qho kev sim. Ib qho 50 µl cov tshuaj tiv thaiv sib xyaw uas muaj qhov tsim nyog NEBuffer, 0.5 µg ntawm calf thymus DNA, thiab 5 los yog 10 µl ntawm kev txwv endonuclease (ntawm kev muag concentration) yog incubated ntawm 37 ° C rau 60 feeb thiab tom qab ntawd ntawm 65 ° C lossis 80 ° C rau 20 feeb.
Ib yam li ntawd, qhov chaw txwv rau BamHI yog dab tsi? BamHI . Kev txwv endonuclease BamH Kuv khi rau ib qho DNA tsis tshwj xeeb. BamH I (los ntawm Bacillus amyloliquefaciens) yog hom II txwv endonuclease, muaj peev xwm paub txog cov ntu luv luv (6 b.p.) ntawm DNA thiab tshwj xeeb cleaving lawv ntawm lub hom phiaj qhov chaw.
Hauv qhov no, kuv puas yuav tsum tau ua kom sov inactivate txwv enzyme?
Inactivation ntawm txwv endonucleases feem ntau tsis yog tsim nyog , tab sis qee zaum nws yuav ua rau kom muaj kev hloov pauv tau zoo.
Dab tsi yog qhov cua sov inactivation?
Kub - kev tsis ua haujlwm ( cua sov mus rau 56 ° C rau 30 feeb) ua tiav rau inactivate ntxiv, ib pawg ntawm cov proteins uas muaj nyob rau hauv sera uas yog ib feem ntawm lub cev tiv thaiv kab mob. Kub kuj tau siv los rhuav tshem mycoplasma hauv cov ntshav.
Pom zoo:
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