Lub hom phiaj ntawm kev siv ddNTPs yog dab tsi?
Lub hom phiaj ntawm kev siv ddNTPs yog dab tsi?

Video: Lub hom phiaj ntawm kev siv ddNTPs yog dab tsi?

Video: Lub hom phiaj ntawm kev siv ddNTPs yog dab tsi?
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DdNTP hais txog Dideoxynucleotides triphosphates uas yog siv nyob rau hauv Sanger dideoxy txoj kev los tsim txawv lengths ntawm DNA strands rau DNA sequencing. Qhov no ua rau kev txiav tawm ntawm DNA polymerisation (lossis DNA elongation) txheej txheem vim tias cov txheej txheem no xav tau pawg 3'-OH txuas ntxiv mus.

Yog li ntawd, vim li cas ddNTPs siv nyob rau hauv sequencing?

Dideoxynucleotides yog chain-elongating inhibitors ntawm DNA polymerase, siv hauv txoj kev Sanger rau DNA ua ke . Cov Dideoxyribonucleotides tsis muaj 3 'hydroxyl pawg, yog li tsis muaj kev sib txuas ntxiv tuaj yeem tshwm sim thaum qhov no dideoxynucleotide nyob rau ntawm cov saw hlau. Qhov no tuaj yeem ua rau kev txiav tawm ntawm DNA ib ntus.

Ib yam li ntawd, puas ddNTPs txuas rau dNTPs? Ib los yog ntau tshaj ntawm cov dNTPs yog radioactively sau npe los pab pom cov khoom replication. Thaum kawg, txhua lub raj tau txais ib qho ntawm plaub qhov tshwj xeeb nucleotides hu ua dideoxynucleotides ( ddNTPs ). Txawm li cas los xij, ib zaug sib koom ua ke, DNA polymerase tsis tuaj yeem txuas ntxiv cov saw hlau vim nws xav tau 3'-OH rau txuas mus rau nucleotide tom ntej.

Ib yam li ntawd ib tug yuav nug, ua li cas ddNTPs nres ib tug sequencing cov tshuaj tiv thaiv?

Thaum muaj me me hauv ua raws li kev xav , dideoxyribonucleoside triphosphates ( ddNTPs ) terminate cov cov tshuaj tiv thaiv kab mob ntawm cov haujlwm sib txawv hauv cov DNA loj hlob. ddNTPs nres ib qho kev sib txuas lus vim lawv: ua rau DNA polymerase poob ntawm cov qauv strand. c.

Yuav siv cov txheej txheem twg uas muaj Dideoxynucleotides?

Sanger sequencing yog ib txoj kev ntawm DNA sequencing raws li kev xaiv kev koom ua ke ntawm cov saw-txiav dideoxynucleotides los ntawm DNA polymerase thaum lub sij hawm nyob rau hauv vitro DNA replication. Tsim los ntawm Frederick Sanger thiab cov npoj yaig hauv xyoo 1977, nws yog qhov dav tshaj plaws siv ua ke txoj kev rau kwv yees li 40 xyoo.

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