Dab tsi yog txwv enzyme tsom xam DNA?
Dab tsi yog txwv enzyme tsom xam DNA?

Video: Dab tsi yog txwv enzyme tsom xam DNA?

Video: Dab tsi yog txwv enzyme tsom xam DNA?
Video: tsis yog nom tub by ntxiag hawj nkauj tawm tshiab 2022 2024, Kaum ib hlis
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Kev txwv enzymes yog cov protein uas zom (txiav) DNA ntawm cov txheej txheem hauv paus. Piv txwv li, a txwv enzyme hu ua EcoRI lees paub qhov sib lawv liag GAATTC.

Ua raws li qhov no saib, kev txwv kev tsom xam DNA yog dab tsi?

A txwv digest yog ib txoj kev siv hauv molecular biology los npaj DNA rau tsom xam los yog lwm yam kev ua. Cov enzymes no hu ua txwv endonucleases lub txwv enzymes, thiab lawv muaj peev xwm cleave DNA molecules ntawm txoj hauj lwm uas tshwj xeeb luv luv sequences ntawm lub hauv paus yog tam sim no.

Tsis tas li ntawd, koj yuav ua li cas thiaj paub qhov txwv enzyme siv los txiav DNA? A txwv enzyme yog a DNA - txiav enzyme uas lees paub qhov chaw tshwj xeeb hauv DNA . Ntau txwv enzymes ua staggered txiav ntawm los yog ze lawv qhov chaw paub, ua tiav nrog ib leeg-stranded overhang. Yog ob DNA molecules muaj qhov sib txuam xaus, lawv tuaj yeem koom nrog enzyme DNA ligase.

Tsis tas li ntawd, lub hauv paus ntsiab lus ntawm kev txwv enzyme tsom xam ntawm chromosomal DNA yog dab tsi?

Kev txwv enzyme cais txawv teb chaws ds DNA ntu ntawm qhov chaw tshwj xeeb ntawm nucleotide ib ntus, feem ntau cov kab ke no yog GAATTC. Nws cleave ntawm A-T nucleotide rau cleavage. Kab mob chromosomes muaj segment ds DNA yog mus tsim Kev txwv enzyme i.e. RE1 thiab RE2 thaum twg bacteriophage yuav kis tau.

Dab tsi enzyme digests DNA?

Kev txwv Endonucleases

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