Video: Reverse transcription PCR siv rau dab tsi?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
RT - PCR ( Rov qab Transscriptase PCR ) RT - PCR yog siv nyob rau hauv kev tshawb fawb lub chaw soj nstuam los kawm gene qhia, piv txwv li hauv kev sim kom paub qhov txawv ntawm exons los ntawm introns, thiab tuaj yeem ua tau. siv soj ntsuam kuaj kab mob genetic kab mob thiab saib xyuas kev kho tshuaj.
Xav txog qhov no, dab tsi xav tau rau kev hloov pauv rov qab?
pib rov qab transcription , thim rov qab transcriptases yuav tsum muaj luv luv DNA oligonucleotide hu ua primer los khi rau nws cov kev sib txuas ntxiv ntawm RNA template thiab ua qhov pib rau kev sib txuas ntawm cov hlua tshiab.
Tsis tas li ntawd, vim li cas nws thiaj tseem ceeb rau kev siv cDNA rau PCR? cDNA muaj nws tus kheej qhov tseem ceeb hauv Polymerase Chain Reaction ( PCR ) txheej txheem. cDNA Nws yog qhov tshwm sim ntawm kev thim rov qab los ntawm cov enzymes hu ua thim rov qab transcriptases. Tam sim no, ua ib daim qauv ntawm cov genomic DNA , qhov no cDNA tuaj yeem ua haujlwm rau lub hom phiaj ntawm tus qauv DNA rau hauv vitro amplification thiab kev soj ntsuam tom qab.
Ua li no, qhov txawv ntawm lub sijhawm PCR thiab rov qab transcriptase PCR yog dab tsi?
RT - PCR yog siv los ua kom nthuav dav qhov hloov pauv ntawm DNA code; QPCR ntsuas qhov amplification. 3. RT - PCR yog rau amplification, thaum qPCR yog rau kom muaj nuj nqis.
Puas yog cDNA muab ob npaug rau?
Tsis zoo li RNA, DNA molecules tuaj yeem yooj yim cloned (cov no hu ua ' cDNA clones ') los ntawm kev ua cDNA ob - tso tseg thiab ligated rau vector DNA. Kev soj ntsuam ib ntus ntawm DNA yog yooj yim dua li ntawm RNA, yog li, cDNA yog daim ntawv tseem ceeb hauv kev tshuaj xyuas RNA, tshwj xeeb ntawm eukaryotic mRNA.
Pom zoo:
Dab tsi yog sub account thiab nws siv rau dab tsi?
Ib tug sub account yog ib tug account segregated nested nyob rau hauv ib tug loj account los yog kev sib raug zoo. Cov nyiaj sib cais no yuav muaj cov ntaub ntawv hauv tsev, ntawv xov xwm, thiab lwm yam ntaub ntawv muaj txiaj ntsig lossis muaj cov nyiaj khaws cia hauv kev nyab xeeb nrog lub txhab nyiaj
Dab tsi yog memo thiab nws siv rau dab tsi?
Ib daim ntawv memo (lossis memorandum, lub ntsiab lus "reminder") feem ntau yog siv rau kev sib txuas lus txoj cai, cov txheej txheem, lossis lwm yam kev lag luam hauv lub koom haum
RTM yog dab tsi nws siv rau dab tsi?
Hauv ib txoj haujlwm tsim kho software, Cov Kev Xav Tau Traceability Matrix (RTM) yog ib daim ntawv uas siv los xyuas kom meej tias tag nrho cov kev xav tau txuas nrog rau cov ntaub ntawv xeem. Qhov no yuav pab kom ntseeg tau tias tag nrho cov kev xav tau yuav raug them rau hauv theem kev sim
Cov chiv yog dab tsi thiab lawv siv rau dab tsi?
Cov neeg ua liaj ua teb tig mus rau cov chiv vim tias cov tshuaj no muaj cov khoom noj xws li nitrogen, phosphorus, thiab potassium. Fertilizers tsuas yog cog cov as-ham siv rau kev ua liaj ua teb kom ntxiv cov ntsiab lus uas yuav tsum tau muaj nyob hauv cov av. Cov chiv tau siv txij thaum pib ua liaj ua teb
Dab tsi yog cov khoom siv ncaj qha kev ua haujlwm thiab kev tsim khoom siv nyiaj siv ua haujlwm?
Hauv cov tuam txhab tsim khoom, cov khoom siv nyiaj siv ua haujlwm suav nrog tag nrho cov nqi tsim khoom tshwj tsis yog cov suav nrog cov khoom siv ncaj qha thiab kev ua haujlwm ncaj qha. Cov nqi tsim khoom siv nyiaj siv ua haujlwm yog cov nqi tsim khoom uas yuav tsum tau tshwm sim tab sis tsis tuaj yeem lossis tsis tuaj yeem taug qab ncaj qha rau cov khoom tshwj xeeb tsim