Video: Vim li cas cov piam thaj siv hauv plasmid DNA cais tawm?
2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Lub hom phiaj ntawm cov kauj ruam no yog txhawm rau nce qhov pib ntim ntawm cov hlwb kom ntau ntxiv plasmid DNA tuaj yeem raug cais rau ib qho kev npaj. Glucose yog ntxiv los ua kom lub siab osmotic sab nraum lub hlwb. Tris yog tus neeg sawv cev buffering siv kom tswj tau qhov pH tsis tu ncua (= 8.0).
Ib yam li ntawd, lub luag haujlwm ntawm cov piam thaj hauv DNA extraction yog dab tsi?
50 hli (millimolar) qabzib qab zib yog ntxiv rau GTE tsis tuaj yeem tswj hwm osmolarity qhov twg cov ntsiab lus nyob sab nraud ntawm lub hlwb nyob ze rau hauv cov hlwb. Qhov no tiv thaiv cell lysis ntxov ntxov, uas tuaj yeem ua rau qis dua DNA yields vim aggregation thiab degradation.
Tsis tas li ntawd, vim li cas NaOH siv hauv DNA rho tawm? Hauv DNA cais los yog rho tawm , NaOH ( Sodium hydroxide ) yog siv raws li alkaline lysis tsis. Nws yeej pab nyob rau hauv dissolving lub cell membrane kom lub puab Cheebtsam ntawm lub cell nrog rau cov DNA tawm tuaj.
Yog li ntawd, lub hom phiaj ntawm plasmid DNA cais tawm yog dab tsi?
Plasmid Isolation . Cov muab cais nyob ib leeg ntawm plasmid DNA los ntawm cov kab mob yog cov txheej txheem tseem ceeb hauv molecular biology thiab yog ib kauj ruam tseem ceeb hauv ntau cov txheej txheem xws li cloning, DNA sequencing, transfection, thiab gene therapy. Cov manipulations yuav tsum tau muab cais nyob ib leeg ntawm siab purity plasmid DNA.
Yuav ua li cas cais plasmid DNA los ntawm E coli?
Cov muab cais nyob ib leeg ntawm plasmid DNA los ntawm E . coli Kev siv cov alkaline lysis yog ib txoj hauv kev zoo tsim. E . coli nrog plasmid yog kab lis kev cai hauv xov xwm nrog tshuaj tua kab mob mus rau lub cell ceev ceev, sau, thiab ces lysed nrog SDS/NaOH tov.
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