Yuav hloov mRNA rau cDNA li cas?
Yuav hloov mRNA rau cDNA li cas?
Anonim

Hauv cov noob caj noob ces, ntxiv DNA ( cDNA ) yog DNA synthesized los ntawm ib leeg-stranded RNA (piv txwv li, messenger RNA ( mRNA ) lossis microRNA (miRNA)) tus qauv hauv cov tshuaj tiv thaiv catalyzed los ntawm enzyme thim rov qab transcriptase. cDNA Feem ntau siv los clone eukaryotic noob hauv prokaryotes.

Kuj paub, vim li cas RNA hloov mus rau cDNA?

Hauv theem tom qab kis mob, thaum tsim tus kab mob tshiab, zoo ib yam cDNA yog siv rau ua tshiab RNA cov lwg me me uas yuav pab ua cov khoom siv caj ces rau tus kab mob tshiab. Ntawm no, tus tswv tsev RNA polymerase tau siv rau tsim nws cov khoom siv caj ces ntawm tus kab mob.

Ib sab saum toj no, cDNA cloning yog dab tsi? cDNA Cloning . mRNA tsis tuaj yeem yog cloned ncaj qha, tab sis DNA ib daim qauv ntawm mRNA tuaj yeem yog cloned . (Hauv qhov no, lub sijhawm cDNA luv luv rau "daim DNA".) Qhov kev hloov pauv no yog ua tiav los ntawm qhov kev txiav txim ntawm thim rov qab transcriptase thiab DNA polymerase. Qhov rov qab transcriptase ua rau ib leeg-stranded DNA luam ntawm mRNA.

Hauv qhov no, puas yog cDNA ntxiv rau mRNA?

thaum cDNA tau tsuas yog los ntawm rov qab transcription ntawm lub mRNA (hais qhia eukaryotic cytosolic mRNA ) feem (piv txwv li, los ntawm poly[dT]n thiab random priming) yog ntxiv DNA ( cDNA ) ua los ntawm qhov hu ua "transcriptome." Eukaryotes muaj kev nkag thiab exons hauv gDNA, thaum prokaryotes tsis ua.

Puas yog cDNA muab ob npaug rau?

Tsis zoo li RNA, DNA molecules tuaj yeem yooj yim cloned (cov no hu ua ' cDNA clones ') los ntawm kev ua cDNA ob - tso tseg thiab ligated rau vector DNA. Kev soj ntsuam ib ntus ntawm DNA yog yooj yim dua li ntawm RNA, yog li, cDNA yog daim ntawv tseem ceeb hauv kev tshuaj xyuas RNA, tshwj xeeb ntawm eukaryotic mRNA.

Pom zoo: