2024 Tus sau: Stanley Ellington | [email protected]. Kawg hloov kho: 2023-12-16 00:17
Hauv cov noob caj noob ces, ntxiv DNA ( cDNA ) yog DNA synthesized los ntawm ib leeg-stranded RNA (piv txwv li, messenger RNA ( mRNA ) lossis microRNA (miRNA)) tus qauv hauv cov tshuaj tiv thaiv catalyzed los ntawm enzyme thim rov qab transcriptase. cDNA Feem ntau siv los clone eukaryotic noob hauv prokaryotes.
Kuj paub, vim li cas RNA hloov mus rau cDNA?
Hauv theem tom qab kis mob, thaum tsim tus kab mob tshiab, zoo ib yam cDNA yog siv rau ua tshiab RNA cov lwg me me uas yuav pab ua cov khoom siv caj ces rau tus kab mob tshiab. Ntawm no, tus tswv tsev RNA polymerase tau siv rau tsim nws cov khoom siv caj ces ntawm tus kab mob.
Ib sab saum toj no, cDNA cloning yog dab tsi? cDNA Cloning . mRNA tsis tuaj yeem yog cloned ncaj qha, tab sis DNA ib daim qauv ntawm mRNA tuaj yeem yog cloned . (Hauv qhov no, lub sijhawm cDNA luv luv rau "daim DNA".) Qhov kev hloov pauv no yog ua tiav los ntawm qhov kev txiav txim ntawm thim rov qab transcriptase thiab DNA polymerase. Qhov rov qab transcriptase ua rau ib leeg-stranded DNA luam ntawm mRNA.
Hauv qhov no, puas yog cDNA ntxiv rau mRNA?
thaum cDNA tau tsuas yog los ntawm rov qab transcription ntawm lub mRNA (hais qhia eukaryotic cytosolic mRNA ) feem (piv txwv li, los ntawm poly[dT]n thiab random priming) yog ntxiv DNA ( cDNA ) ua los ntawm qhov hu ua "transcriptome." Eukaryotes muaj kev nkag thiab exons hauv gDNA, thaum prokaryotes tsis ua.
Puas yog cDNA muab ob npaug rau?
Tsis zoo li RNA, DNA molecules tuaj yeem yooj yim cloned (cov no hu ua ' cDNA clones ') los ntawm kev ua cDNA ob - tso tseg thiab ligated rau vector DNA. Kev soj ntsuam ib ntus ntawm DNA yog yooj yim dua li ntawm RNA, yog li, cDNA yog daim ntawv tseem ceeb hauv kev tshuaj xyuas RNA, tshwj xeeb ntawm eukaryotic mRNA.
Pom zoo:
Yuav ua li cas yog qhov tsawg kawg nkaus pob zeb npog nyob rau hauv mm ntawm ib tug cam khwb cia nyob rau hauv qhov chaw pob zeb tso tawm tsam thiab mus tas li raug rau lub ntiaj teb?
Table-1: Yam Tsawg Npog Thickness rau Cast-in-Place Qhob Hom kev tsim ua vaj tse hla, hli Qhob nrum tiv thaiv thiab txuas mus tas li nrog hauv av 75 Pob zeb sib chwv nrog av lossis dej No. 19 txog No. 57 tuav 50 No. 16 bar thiab me dua 40
Kuv yuav hloov li cas los ntawm kev sib koom ua tswv cuab rau cov neeg xaum nyob ua ke?
Ua kom tiav Daim Ntawv Sau Npe Av SEV - Daim Ntawv Thov nkag mus rau Daim Ntawv Thov Kev txwv ntawm kev sib koom ua ke los ntawm kev pom zoo lossis kev ceeb toom. Koj tuaj yeem siv SEV nrog cov pov thawj txhawb nqa los hloov cov tswv cuab lub npe rau cov neeg xaum nyob ua ke yam tsis muaj lwm Tus Neeg Xib Hwb Kev Pom Zoo pom zoo
Yuav ua li cas hloov lub teeb recessed rau ib dai pendant?
Cov Khoom Siv. Muas cov khoom siv hloov kho kom rov zoo, qab nthab nruab nrab thiab teeb pom kev zoo. Xaiv Recessed Light los Hloov. Xaiv qhov chaw los dai lub teeb tshiab. Tshem Tawm Cov Teeb Tsom Teeb. Nruab Recessed Fixture Converter. Npaj Kho Tshiab. Txuas Txuas. Kev sib txuas. Daim kab xev Ceiling Medallion
Kuv yuav hloov qhov chaw nres nkoj li cas rau Splunk?
Txhawm rau hloov cov chaw nres nkoj los ntawm lawv qhov chaw teeb tsa: Nkag mus rau Splunk Web raws li tus neeg siv admin. Nyem Chaw nyob rau sab saum toj-sab xis ntawm lub interface. Nyem rau Server chaw txuas hauv ntu System ntawm qhov screen. Nyem General nqis. Hloov tus nqi rau Management chaw nres nkoj lossis Web chaw nres nkoj, thiab nyem Txuag
Yuav ua li cas yog lub npe rau ib tug monetary system nyob rau hauv uas daim ntawv nyiaj thiab npib yog sib npaug zos rau tus nqi ntawm ib tug tej yam nyiaj ntawm kub?
Tus qauv kub yog ib qho nyiaj txiag uas lub teb chaws lub txiaj los yog cov ntawv nyiaj muaj nqis ncaj qha mus rau kub. Nrog tus qauv kub, lub teb chaws tau pom zoo los hloov cov ntawv nyiaj rau hauv cov nyiaj kub kub